鬼针草水提物抗伪狂犬病毒作用研究  

Effect of aqueous extract of Bidens pilosa L.on Pseudorabies virus

作　　者：郭志军 谢慧凡 吴其文 陈洪博[1,3] 邱龙新 GUO Zhijun;XIE Huifan;WU Qiwen;CHEN Hongbo;QIU Longxin(School of Life Science,Longyan University,Longyan,Fujian 364012,China;Fujian Longyan Minxiong Biotechnology Co.,Ltd,Longyan,Fujian 364012,China;Fujian Provincial Universities Key Laboratory of Preventive Veterinary Medicine and Biotechnology,Longyan,Fujian 364012,China)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]福建龙岩闽雄生物科技股份有限公司,福建龙岩364012 [3]预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩364012

出　　处：《西北农林科技大学学报（自然科学版）》2022年第5期18-24,35,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中央引导地方科技发展专项(2019L3011);龙岩市奇迈基金项目(2018LYQM0201);预防兽医学与生物技术福建省高等学校重点实验室开放课题(2019KF01)。

摘　　要：【目的】研究鬼针草水提物(BPE)的体外抗伪狂犬病毒(Pseudorabies virus,PrV)作用。【方法】制备640 mg/mL(以生药计)的BPE,检测其对仓鼠肾上皮细胞(BHK-21)的毒性(半数中毒浓度(TC_(50)))及对PrV体外抗性(半数抑制浓度(EC_(50)))和治疗指数(TI)。采用CCK-8法检测BPE抗PrV的机制。采用间接免疫荧光法检测BPE对gB蛋白表达和PrV所致细胞凋亡的影响,采用实时荧光定量PCR方法检测BPE对PrV gB基因表达的影响。【结果】BPE对BHK-21细胞的TC_(50)为28.7 mg/mL,对PrV的EC_(50)为5.16 mg/mL,TI为5.56。BPE抗PrV的机制为抑制PrV增殖和灭活PrV。BPE可抑制PrV诱导的BHK-21细胞凋亡,抑制PrV gB基因和蛋白的表达。【结论】BPE具有明显的体外抗PrV活性。【Objective】This study aimed to investigate the protective effect of aqueous extract of Bidens pilosa L.(BPE)on Pseudorabies virus(PrV)in vitro.【Method】The cytotoxicity and anti-PrV activity of BPE at concentration of 640 mg/mL on BHK-21 cells were detected by CCK-8,and the anti-PrV mechanism of BPE was investigated by CCK-8.The TC_(50),EC_(50) and TI were determined.The indirect immunofluorescence was used to detect the effect of BPE on PrV-induced apoptosis and expression of PrV gB protein,and the real-time fluorescence quantitative PCR was used to detect the expression of PrV gB gene.【Result】The TC_(50) of BPE in BHK-21 cells was 28.7 mg/mL,the IC_(50) of PrV was 5.16 mg/mL,and the TI was 5.56.The mechanism of anti-PrV was based on inhibiting PrV proliferation and inactivating PrV.BPE might protect PrV-infected BHK-21 cells through suppressing expression of PrV gB gene and protein(P<0.01)and inhibiting PrV-induced apoptosis of BHK-21 cells.【Conclusion】BPE had significant anti-PrV activity in vitro.

关 键 词：鬼针草水提物 伪狂犬病毒 BHK-21细胞 

分 类 号：S853.7[农业科学—临床兽医学] S852.659.1[农业科学—兽医学]