雷公藤中倍半萜生物碱类化学成分的研究  

Chemical constituents of sesquiterpene alkaloids from Tripterygium wilfordii

作　　者：闫建功 王一竹 吴先富[2] 陈明慧 郑玉光[1] 王亚丹[2] 马双成[2] YAN Jian-gong;WANG Yi-zhu;WU Xian-fu;CHEN Ming-hui;ZHENG Yu-guang;WANG Ya-dan;MA Shuang-cheng(Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;National Institutes for Food and Drug Control,Beijing 100050,China;Department of Pharmacy,the First Hospital of Tsinghua University,Beijing 100016,China)

机构地区：[1]河北中医学院河北省中药炮制技术创新中心,石家庄050200 [2]中国食品药品检定研究院,北京100050 [3]清华大学第一附属医院药学部,北京100016

出　　处：《中草药》2022年第7期1933-1938,共6页Chinese Traditional and Herbal Drugs

基　　金：中国食品药品检定研究院中药所学科建设项目:雷公藤及制剂的质量研究(1020050090113)。

摘　　要：目的对雷公藤Tripterygium wilfordii乙醇提取物氯仿部位中的倍半萜生物碱类成分进行研究。方法采用酸提碱沉法,结合中性氧化铝、十八烷基硅烷键合硅胶(ODS)及半制备高效液相等多种色谱技术进行分离纯化,并运用1D-NMR、2D-NMR、MS、ECD等波谱学方法对化合物进行结构鉴定。结果从雷公藤乙醇提取物氯仿部位中分离得到5个倍半萜生物碱类成分,分别鉴定为(1S,4S,5R,6R,7R,8S,9S,10S)-1,8,11-triacetoxy-7-nicotinoyloxy-4,5-dihydroxy-dihydroagarofuran(1)、triptersinine U(2)、macroregeline F(3)、雷公藤宁碱B(4)和triptonine B(5)。结论化合物1为新化合物,命名为雷公藤司宁Z15;化合物3为首次从该植物中分离得到。Objective To study the sesquiterpene alkaloids from the chloroform fraction of the ethanolic extract of Tripterygium wilfordii.Methods Compounds were isolated and purified by acid extraction and alkaline precipitation method,combined with various chromatographic techniques such as neutral alumina,octadecylsilane bonded silica(ODS)and semi-preparative high performance liquid chromatography.Their structures were elucidated by means of various spectroscopic techniques such as 1 D-NMR,2 D-NMR,MS,and ECD.Results A total of five sesquiterpene alkaloids were isolated from the chloroform fraction of the ethanolic extract of T.wilfordii,and identified as(1 S,4 S,5 R,6 R,7 R,8 S,9 S,10 S)-1,8,11-triacetoxy-7-nicotinoyloxy-4,5-dihydroxy-dihydroagarofuran(1),triptersinine U(2),macroregeline F(3),wilfornine B(4),and triptonine B(5).Conclusion Compound 1 is a new sesquiterpene alkaloid named triptersinine Z15,and compound 3 is isolated from T.wilfordii for the first time.

关 键 词：雷公藤 倍半萜生物碱 雷公藤宁碱B 雷公藤司宁Z15 macroregeline F 

分 类 号：R284.1[医药卫生—中药学]