水稻耐低氮基因OsTPP6的鉴定及功能分析  

Identification and characterization analysis of low nitrogen tolerance gene OsTPP6 in rice

作　　者：任秋韵 田云录[1] 刘晓岚 余珺 唐伟杰 陈高明 林之希 李静 王春明[1] 万建民 REN Qiuyun;TIAN Yunlu;LIU Xiaolan;YU Jun;TANG Weijie;CHEN Gaoming;LIN Zhixi;LI Jing;WANG Chunming;WAN Jianmin(State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Collaborative Innovation Centre forModern Crop Production,Nanjing Agricultural University,Nanjing 210095,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心,江苏南京210095 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《南京农业大学学报》2022年第3期431-441,共11页Journal of Nanjing Agricultural University

基　　金：国家重点研发计划项目(2021YFF1000400);国家自然科学基金项目(31872806);中央高校基本科研业务费专项资金(JCQY201901)。

摘　　要：[目的]本文旨在研究新的高效利用氮素基因,以改良水稻品种。[方法]通过对117份水稻地方品种的耐低氮表型进行全基因组关联分析(GWAS),鉴定了1个水稻氮素利用相关基因OsTPP6,对野生型‘Dongjin’和OsTPP6 T-DNA插入突变体进行氮素利用相关基因的差异表达,及对硝态氮的响应和硝态氮^(15)N吸收速率进行分析;在苗期和大田对野生型和突变体进行表型验证和籽粒表型观察,并分析其淀粉代谢相关基因表达差异和籽粒淀粉理化性质。[结果]低浓度硝态氮显著诱导了OsTPP6的表达。与野生型相比,OsTPP6 T-DNA插入突变体与硝态氮利用相关的基因均显著上调表达,T-DNA插入突变体硝态氮响应高于野生型。低氮田中,OsTPP6 T-DNA插入突变体相较于野生型有效分蘖数、结实率、千粒重均下降。此外,OsTPP6 T-DNA插入突变体籽粒表现为高垩白,与淀粉代谢相关的基因均显著上调表达。[结论]OsTPP6参与硝态氮利用相关途径,影响籽粒淀粉代谢。[Objectives]In this paper,we aimed to indentify genes which are able to enhance nitrogen-use efficiency for rice breeding improvement.[Methods]We identified a nitrogen-use efficiency(NUE)related gene OsTPP6 via genome wide association study(GWAS)using NUE related traits from 117 rice landraces,and then tested the response of OsTPP6 to nitrate treatment.We analysed the expression levels of nitrogen utilization-related genes in wild type‘Dongjin’and OsTPP6 T-DNA mutation,and detected the difference of ^(15)N uptake rate between wild type and OsTPP6 T-DNA mutation using ^(15)NO_(3)^(-).Furthermore,we determined the agronomic traits,seed appearance phenotypes and physical and chemical properties,the expression levels of starch metabolism-related genes of wild type and OsTPP6 T-DNA mutation.[Results]OsTPP6 was induced by low concentration of nitrate.Nitrate-related genes were up-regulated in OsTPP6 T-DNA mutation compared with the wild type.In low nitrogen field,OsTPP6 T-DNA mutation showed decreased effective panicle numbers,seed setting rate and 1000-grain weight.In addition,the grain of OsTPP6 T-DNA mutation showed high chalkiness and significantly up-regulated of starch metabolism-related genes.[Conclusions]OsTPP6 participated in the pathways of nitrate utilization and affected starch metabolism.

关 键 词：水稻 OsTPP6 硝态氮 氮素利用效率 籽粒淀粉 

分 类 号：S511.032[农业科学—作物学]