海金沙卵发生的组织化学研究  

Histochemical Study on Oogenesis in Lygodium japonicum(Thunb.)Sw.

作　　者：郭严冬 张明霞[1] 周义峰[1] 戴锡玲[2] 王全喜[2] 曹建国[2] GUO Yandong;ZHANG Mingxia;ZHOU Yifeng;DAI Xiling;WANG Quanxi;CAO Jianguo(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014;College of Life Science,Shanghai Normal University,Shanghai 200234)

机构地区：[1]江苏省中国科学院植物研究所,南京210014 [2]上海师范大学生命科学学院,上海200234

出　　处：《植物研究》2022年第3期352-357,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30970267);上海市教委重点项目(12ZZ128)。

摘　　要：采用组织化学方法对海金沙(Lygodium japonicum(Thunb.)Sw.)卵发生进行了研究。高碘酸-锡夫反应(PAS反应)结果显示:颈沟和腹沟细胞外产生的黏性物质为多糖类物质;在卵细胞发生早期颈卵器细胞内均含有质体,且淀粉粒含量丰富,随着颈卵器的发育,卵细胞以及颈沟和腹沟细胞内淀粉粒数量和体积均逐渐减少,最后退化消失。苏丹黑B反应结果显示:海金沙颈沟和腹沟细胞外产生的黏性物质也含有脂类物质,而卵细胞内并未有明显脂类物质产生;卵细胞质膜处也并没有糖类或脂类物质的积累。本研究从组织化学角度佐证了海金沙的特殊性,即既有原始蕨类的特征,又有进化蕨类的特征。The oogenesis of Lygodium japonicum(Thunb.)Sw.was studied by histochemical method in thisstudy.The results of PAS reaction showed that the extracellular viscous substances of neck canal and ventralcanal cells were polysaccharides;there were plastids and rich starch granules in cell of archegonium in the earlystage of oogenesis,with the development of archegonium,the number and volume of starch granules in the eggand neck canal and ventral canal cells gradually decreased,and finally degenerated and disappeared.Theresults of Sudan black B reaction showed that the extracellular viscous substances of neck canal and ventralcanal cells of L.japonicum also contain lipids,but there was no obvious lipids production in egg cells.Comprehensive studies showed that there was no accumulation of carbohydrates or lipids in the plasmamembrane of egg cells.From the perspective of histochemistry,this study confirmed the particularity of L.japonicum,it had the characteristics of both primitive ferns and evolutionary ferns.

关 键 词：海金沙 卵发生 组织化学 PAS反应 苏丹黑B反应 

分 类 号：Q949.36[生物学—植物学] Q24