VEGF对缺氧复氧小鼠海马神经元细胞线粒体凋亡的抑制作用  

Inhibitory effect of VEGF on mitochondrial apoptosis in hippocampal neurons of mice with hypoxia-reoxygenation

作　　者：张业森[1] 尚毓淳 姜之全[1] 苏贺先[1] 赵永轩[1] Zhang Yesen;Shang Yuchun;Jiang Zhiquan;Su Hexian;Zhao Yongxuan(Department of Neurosurgery,The First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,Anhui,China)

机构地区：[1]蚌埠医学院第一附属医院神经外科,安徽蚌埠233004

出　　处：《右江民族医学院学报》2022年第2期190-194,共5页Journal of Youjiang Medical University for Nationalities

基　　金：蚌埠医学院自然科学重点项目(BYKY2019093ZD)。

摘　　要：目的研究血管内皮生长因子(VEGF)对缺氧复氧小鼠海马神经元细胞系HT22细胞凋亡与线粒体动力相关蛋白1(Drp1)表达情况的影响。方法取对数生长期的HT22细胞,随机分3组:正常对照组(Control)、缺氧复氧组(Model)、Model+VEGF组(Model+VEGF)。除Control组外,其余各组细胞在缺氧缺糖处理6h后,进行复氧复糖继续培养18h,Model+VEGF组在缺氧复氧结束后加入VEGF 164处理24h。倒置显微镜观察各组细胞形态,Hoechst33258染色及流式细胞术检测细胞凋亡情况,Western Blot检测细胞中Drp1的表达情况。结果Control组细胞呈两级或者多级,胞体明显,细胞更为短小与饱满,细胞交织成片连接在一起;Model组细胞皱缩,胞体变圆,细胞显微镜下观察细胞突触变长,细胞间连接减少;Model+VGEF组细胞形态与Model组相比有所缓解;与Control组相比,Model组细胞凋亡与Drp1蛋白表达量显著增加(P<0.05);与Model组相比,Model+VEGF组细胞凋亡率与Drp1蛋白表达量明显下降(P<0.05)。结论VEGF可能通过抑制细胞线粒体凋亡对缺氧复氧的HT22细胞起到保护作用。Objective To investigate the effect of vascular endothelial growth factor(VEGF)on the apoptosis of cell line HT22and the expression of mitochondrial dynamic-related protein 1(Drp1)in hippocampal neurons of mice with hypoxia-reoxygenation.Methods HT22cells in the logarithmic growth phase were randomly divided into 3groups:the control group(Control),hypoxia-reoxygenation group(Model),and Model+VEGF group.Except for the control group,cells in other groups were treated with deficiency of hypoxia and glucose for 6h,and then co-cultured with hypoxia-reoxygenation for 18h.The Model+VEGF group was treated with VEGF 164for 24hafter co-culture with hypoxia-reoxygenation.The cell morphology of each group was observed under inverted microscope.Apoptosis was detected by Hoechst 33258staining and flow cytometry,and Drp1expression by Western Blot.Results The control group had two or more levels of cells with obvious bodies which were short and full,interwoven into slices and joined together.Cells in the Model group shriveled,the cell body becoming round,with cell synapses becoming longer and intercellular connections decreasing under the cell microscope.Compared with that of the Model group,the cell morphology of the Model+VGEF group alleviated.Compared with the control group,the Model group had significantly increased apoptosis rate and expression of Drp1(P<0.05).Compared with the Model group,the Model+VEGF group had significantly decreased apoptosis rate and expression of Drp1(P<0.05).Conclusion VEGF may protect HT22cells of hypoxia-reoxygenation by inhibiting mitochondrial apoptosis.

关 键 词：血管内皮生长因子 HT22细胞 缺氧复氧 线粒体动力相关蛋白1 

分 类 号：R651.15[医药卫生—外科学]