茄子和托鲁巴姆ZIP5和ZIP11基因对锌/镉毒害的响应分析  

Response analyses of ZIP5 and ZIP11 genes in Solanum melongena and Solanum torvum to zinc/cadmium toxicity

作　　者：吴非 刘飞[1] 张苹 李栋 孙亮亮 徐进[1] Wu Fei;Liu Fei;Zhang Ping;Li Dong;Sun Liangliang;Xu Jin(College of Horticulture,Shanxi Agricultural University,Taigu 030801,China;College of Resources and Environment Sciences,China Agricultural University,Beijing 100091)

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]中国农业大学资源与环境学院,北京100091

出　　处：《山西农业大学学报（自然科学版）》2022年第2期37-44,共8页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31772383)。

摘　　要：[目的]本文旨在对比研究锌(Zn)或镉(Cd)毒害下,茄子砧木托鲁巴姆(Solanum torvum Sw.)中StZIP5与StZIP11基因和茄子(Solanum melongena L.)中SmZIP5与SmZIP11基因表达水平的差异,探讨StZIP5与StZIP11基因对植物Zn/Cd毒害耐受性的影响,为初步探明StZIP5和StZIP11基因的功能提供理论依据。[方法]生长4周的托鲁巴姆和茄子幼苗在Hoagland营养液中分别添加200μmol·L^(-1)Zn或10μmol·L^(-1)Cd处理,分析了StZIP5/11和SmZIP5/11基因对Zn/Cd毒害的响应。以拟南芥野生型Col-0、zip5和zip11突变体及异源表达StZIP5或StZIP11基因的拟南芥转基因植株为材料,分析了Zn/Cd毒害下幼苗主根生长量。[结果]StZIP5与SmZIP5,以及StZIP11与SmZIP11之间的蛋白序列展示出高度相似性。Zn毒害抑制托鲁巴姆根、茎和叶中StZIP5基因表达,但诱导了茄子根中SmZIP5和SmZIP11基因表达。Cd毒害诱导托鲁巴姆和茄子根、茎和叶中ZIP11基因显著表达,并且3 d时SmZIP5基因在茄子根中被诱导表达。然而,托鲁巴姆茎中StZIP5基因表达在Cd毒害3 d时被显著抑制。与拟南芥Col-0相比,atzip5或atzip11突变体增强主根对Zn/Cd毒害的耐受性。相反,过表达StZIP5或StZIP11基因增加了拟南芥转基因植株对Zn/Cd毒害的敏感性。[结论]初步揭示了Zn/Cd毒害下托鲁巴姆和茄子中ZIP5与ZIP11基因的功能差异。托鲁巴姆StZIP5或StZIP11基因在拟南芥中异源表达提高了拟南芥主根对Zn/Cd毒害的敏感性。[Objective]TThe present study aimed to compare the gene expression patterns of StZIP5 and StZIP11 in eggplant rootstock Solanum torvum and SmZIP5 and SmZIP11 genes in eggplant(Solanum melongena L.)in response to zinc(Zn)or cadmium(Cd)toxicity,and to explore the effects of StZIP5/11 genes on Zn/Cd toxicity in plants.These results will provide a theoretical basis for further elucidating the biological function of StZIP5 and StZIP11 genes.[Methods]Four-week-old seedlings of each species were treated with 200μmol·L^(-1)Zn or 10μmol·L^(-1)Cd in Hoagland solution.The gene expressions of StZIP5/11 and SmZIP5/11 under Zn/Cd stresses were investigated.The growth of the main root system of seedlings under Zn/Cd stress was analyzed using Arabidopsis wild-type Col-0,zip5 and zip11 mutant,and the transgenic plants heterologous overexpressing StZIP5 or StZIP11 genes as the materials.[Results]The protein sequences between StZIP5 and SmZIP5 and between StZIP11 and SmZIP11 showed high similarity.Zn toxicity inhibited the expression of StZIP5 in roots,stems and leaves of S.torvum seedlings,but induced the expression of SmZIP5 and SmZIP11 genes in the roots of eggplant seedlings.Cd toxicity induced the expression of ZIP11 gene in the roots,stems and leaves of S.torvum and eggplant,and the expression of SmZIP5 gene was markedly induced in eggplant roots at the 3rd day.However,the expression of StZIP5 gene in S.torvum stem was markedly inhibited at the 3rd day by Cd toxicity.Compared with Arabidopsis Col-0,the atzip5 and atzip11 mutants enhanced the tolerance of main root system to Zn or Cd toxicity.On the contrary,overexpressing of StZIP5 or StZIP11 genes increased the susceptibility of Arabidopsis transgenic plants to Zn or Cd toxicity.[Conclusion]These results revealed the functional differences between ZIP5 and ZIP11 in S.torvum and eggplant responding to Zn/Cd toxicity.Heterologous expression of StZIP5 or StZIP11 genes of S.torvum in Arabidopsis increased the sensitivity of Arabidopsis root growth to Zn/Cd toxicity.

关 键 词：锌转运蛋白 托鲁巴姆 茄子 基因表达 锌/镉毒性 

分 类 号：S641.1[农业科学—蔬菜学]