循环血Septin 9基因甲基化检测在结直肠癌筛查中的应用价值  

Role of methylation status of serum Septin 9 gene in colorectal cancer screening

作　　者：徐世赟 张洪来 刘志鹏 杨芳花 朱小龙 张维胜[1,2] 杨熊飞[1,2] 胡东平[1,2] Xu Shi-yun;Zhang Hong-lai;Liu Zhi-peng;Yang Fang-hua;Zhu Xiao-long;Zhang Wei-sheng;Yang Xiong-fei;Hu Dong-ping(Department of Anorectal Surgery,,Gansu Provincial Hospital,Lanzhou 730000,China;Clinical Research Center for Anorectal Diseases of Gansu Province,Gansu Provincial Hospital,Lanzhou 730000,China;Department of Clinical Medicine,Ningxia Medical University,Yinchuan 750000,China;The First Department of Internal Medicine,Longxi County People's Hospital,Longxi 748100,Gansu,China;Department of Clinical Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;不详)

机构地区：[1]甘肃省人民医院肛肠科,甘肃兰州730000 [2]甘肃省人民医院甘肃省肛肠疾病临床医学研究中心,甘肃兰州730000 [3]宁夏医科大学临床医学研究生院,宁夏银川750000 [4]陇西县人民医院内一科,甘肃陇西748100 [5]甘肃中医药大学临床医学研究生院,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2022年第4期35-38,44,共5页Journal of Lanzhou University（Medical Sciences）

基　　金：甘肃省自然科学基金资助项目(18JR3RA055);兰州市科技发展指导性计划资助项目(2017-ZD-42);甘肃省人民医院院内科研基金资助项目(21GSSYB-22)。

摘　　要：目的研究循环血Septin 9基因甲基化在结直肠癌筛查中的检出率及术后监控中的临床意义。方法选取甘肃省人民医院2018年1月-2019年1月入院结直肠癌患者125例为实验组,74例非肿瘤患者为对照组,检测外周血Septin 9基因甲基化、癌胚抗原(CEA)、CA199、CA724,统计4项检查指标的诊断灵敏度、特异度、总符合率,观察结直肠癌患者术后4项检查指标阳性率。结果Septin 9基因甲基化阳性检出率实验组较对照组显著升高(P<0.05)。实验组检测中Septin 9基因甲基化诊断灵敏度较其他3项检查指标显著增高(P<0.05),4项检测指标总符合率对比Septin 9为最高(P<0.05)。结论循环血Septin 9基因甲基化检测较CEA、CA199、CA724在结直肠癌诊断中具有更高诊断效能,可用于结直肠癌筛查以及疗效评估。Objective To study the value of peripheral blood Septin 9 gene methylation status in colorectal cancer screening.Methods 125 patients with colorectal cancer admitted to Gansu Provincial Hospital from January 2018 to January 2019 were enrolled in the experimental group and 74 ordinary patients in the control group.The two groups were tested for the methylation of serum Septin 9 gene,CEA,CA199 and CA724;the colorectal cancer patients in the experimental group and the control group were analyzed,the four indexes were counted and observed.Results The positive detection rate of Septin 9 gene methylation was significantly higher in the experimental group than in the control group(P<0.05).The diagnostic sensitivity of the methylation of Septin 9 gene in the colorectal cancer patients was significantly higher than that of the other three indexes(P<0.05).The total coincidence rates of the four indexes,Septin 9 gene was the highest(P<0.05).Conclusion Compared with CEA,CA199,and CA724,the detection of the peripheral blood Septin 9 gene methylation is more effective in the diagnosis of colorectal cancer and can be used in colorectal cancer screening.

关 键 词：外周血Septin 9基因甲基化检测 结直肠癌 临床筛查 肿瘤标志物 

分 类 号：R446.1[医药卫生—诊断学] R735.3[医药卫生—临床医学]