HIV-1感染者外周血Tfh分泌CXCL13水平与浆母细胞分化的相关性分析  

Correlation between the level of CXCL13 secretion by circulating T follicular helper cell and the differentiation of plasmablasts in HIV-1 infected patients

作　　者：王蕊[1] 李珍[1] 计云霞[1] 张欣[1] 粟斌[1] 张彤[1] 陆小凡[1] WANG Rui;LI Zhen;JI Yunxia;ZHANG Xin;SU Bin;ZHANG Tong;LU Xiaofan(Beijing Key Lab for HIV/A IDS Research,Clinical and Research Center for Infectious Diseases,Beijing Youan Hospital,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京佑安医院感染性疾病临床研究中心艾滋病研究北京市重点实验室,北京100069

出　　处：《中国艾滋病性病》2022年第4期392-396,共5页Chinese Journal of Aids & STD

基　　金：北京市医管中心人才培养“青苗”“登峰”计划(QML20181702,DFL20191701);国家自然科学基金项目(82072294,81772165);艾滋病研究北京市重点实验室(BZ0089)。

摘　　要：目的了解HIV-1不同感染阶段外周血滤泡辅助性T细胞(cTfh)分泌CXCL13的水平及与浆母细胞分化的相关性。方法选取25名HIV-1感染者和21名HIV-1阴性的健康对照者(HC),分别采用该25名感染者急性期感染(AHI)阶段和慢性期感染(CHI)阶段以及HC的外周血单个核细胞,利用流式染色的方法,检测CXCL13+cTfh及浆母细胞(PB)的比例。同时,用ELISA方法检测AHI、CHI和HC血浆中CXCL13浓度。结果AHI和CHI阶段CXCL13+cTfh[AHI vs.HC:(7.71±3.53)vs.(2.08±1.14),P<0.001;CHI vs.HC:(8.52±3.74)vs.(2.08±1.14),P<0.001]和PB[AHI vs.HC:(2.88±2.37)vs.(0.52±0.17),P<0.001;CHI vs.HC:(1.90±1.49)vs.(0.52±0.17),P<0.001)]的比例均显著高于HC。CHI阶段,CXCL13+cTfh比例均与血浆CXCL13浓度(r=0.44,P=0.039)和PB比例(r=0.44,P=0.034)呈正相关。结论CHI阶段CXCL13+cTfh比例的升高,可能是导致PB过度分化的因素。Objective To understand the correlation between the level of CXCL13 secretion by circulating T follicular helper cells(cTfh)and the differentiation of plasmablasts(PB)in acute-phase and consequent chronic-phase of HIV-1 infection.Methods 25 HIV-1 infected patients and 21 HIV-1 negative healthy controllers(HC)were enrolled by the study.Multicolor flow cytometry was used for the ratio analysis of CXCL13+cTfh and PB by using PBMC samples of acute HIV-1 infection(AHI),consequently chronic HIV-1 infection(CHI),and HC.The concentration of plasma CXCL13was measured by ELISA.Results The ratios of CXCL13+cTfh(AHI vs.HC:7.71±3.53 vs.2.08±1.14,P<0.001;CHI vs.HC:8.52±3.74 vs.2.08±1.14,P<0.001)and PB(AHI vs.HC:2.88±2.37 vs.0.52±0.17,P<0.001;CHI vs.HC:1.90±1.49 vs.0.52±0.17,P<0.001)were elevated in both AHI and CHI phases.In CHI stage,the ratio of CXCL13+cTfh was positively correlated with plasma CXCL13 concentration(r=0.44,P=0.039)and PB ratio(r=0.44,P=0.034).Conclusions The increased ratio of CXCL13+cTfh in CHI stage may be a factor leading to excessive differentiation of PB.

关 键 词：1型艾滋病病毒 滤泡辅助性T细胞 浆母细胞 CXCL13 

分 类 号：R373.9[医药卫生—病原生物学]