三种TP-IgM检测方法在先天梅毒早期诊断中的应用  被引量:1

Application of Three Methods in Early Diagnosis of Congenital Syphilis

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作  者:张鹏 刘意[2] ZHANG Peng;LIU Yi(Yanjing Medical School,Capital Medical University,Beijing 101300;Beijing You'an Hospital Affiliated to Capital Medical University,Beijing 100069)

机构地区:[1]首都医科大学燕京医学院,北京101300 [2]首都医科大学附属北京佑安医院,北京100069

出  处:《中国医疗器械信息》2022年第7期43-45,共3页China Medical Device Information

基  金:首都医科大学基础-临床科研合作基金(项目名称:梅毒螺旋体ELISA S/CO比值、TPPA、RPR在先天梅毒实验室诊断中的联合应用,项目编号:9JL61)。

摘  要:目的:评价荧光梅毒螺旋体抗体吸收试验(Fluorescent Treponemal Antibody Absorption Test,FTA-ABS-IgM)、梅毒螺旋体免疫蛋白印迹法(Western Blotting,TP-IgM-WB)和酶联免疫吸附试验(Enzyme-Linked Immunosorbent Assay,TP-IgM-ELISA)在先天梅毒早期诊断中的应用。方法:对300例疑似先天性梅毒婴儿血清标本进行FTA-ABS-IgM、TP-IgM-WB和TP-IgM-ELISA检测,并每隔3个月对三种方法结果进行随访至18个月,根据随访的最终结果回顾性分析三种方法在先天性梅毒早期诊断中的价值。结果:300例感染梅毒的孕妇分娩的婴儿中18例诊断为先天梅毒患儿。血清学检测中,FTA-ABS-IgM方法诊断特异性为100%,敏感性94.44%;TPIgM-WB方法诊断特异性为100%,敏感性为83.33%;TP-IgM-ELISA诊断特异性为96.45%,敏感性为72.22%。经统计分析,三种检测方法之间比较差异有统计学意义(χ^(2)=23.40,P<0.05)。在非先天性梅毒婴儿组的转归分析中,ELISA法转阴时间中位数为12个月。结论:FTA-ABS-IgM试验比其他方法具有较高的敏感性和特异性,可以作为先天梅毒早期的诊断检测方法。Objective:To evaluate the Fluorescent Treponemal Antibody Absorption Test(FTA-ABS-IgM),Western Blotting(TP-IgM-WB)and Enzyme-Linked Immunosorbent Test Enzyme-Linked Immunosorbent Assay(TP-IgM-ELISA)in the early diagnosis of congenital syphilis.Methods:FTA-IgM,TP-IgM-WB and TP-IgM-ELISA were performed on serum samples of 300 infants suspected of congenital syphilis,and the results of the three methods were followed up every three months to 18 months.The final results of the retrospective analysis of the value of three methods in the early diagnosis of congenital syphilis.Results:Among 300 infants born to syphilis-infected pregnant women,18 were diagnosed with congenital syphilis.In serological detection,the diagnostic specificity of FTA-ABS-IgM method was 100% and the sensitivity was 94.44%;the diagnostic specificity of TP-IgMWB method was 100% and the sensitivity was 83.33%;the diagnostic specificity of TP-IgM-ELISA was 96.45%%,the sensitivity was 72.22%.After statistical analysis,there were significant differences among the three detection methods(χ^(2)=23.40,P<0.05).In the outcome analysis of the infant group without congenital syphilis,the median time to ELISA negative was 12 months.Conclusion:FTA-ABS-IgM has higher specificity and sensitivity than other methods,and can be used as early diagnostic index of congenital syphilis.

关 键 词:先天性梅毒 IGM抗体 荧光梅毒螺旋体抗体吸收试验 

分 类 号:TH776[机械工程—仪器科学与技术]

 

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