防风通圣丸改善多囊卵巢综合征大鼠胰岛素抵抗的作用研究  

Fangfeng Tongsheng Pill Alleviating IR in Rats with PCOS

作　　者：韩方 张荧 蒯顾平 任青玲[1] 胡荣魁[1] HAN Fang;ZHANG Ying;KUAI Guping;REN Qingling;HU Rongkui(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;School of Medicine&Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学医学院·整合医学学院,江苏南京210023

出　　处：《中医药学报》2022年第5期24-28,共5页Acta Chinese Medicine and Pharmacology

基　　金：江苏省重点研发项目(BE2019770);江苏省333工程项目(2016Ⅲ-3288);江苏省六大人才高峰项目(WSN-044);江苏省中医药科技发展专项(2020ZX04);江苏省人口学会开放项目(JSPA2019016);江苏省妇幼健康研究会项目(JSFY202003)。

摘　　要：目的:探讨防风通圣丸改善多囊卵巢综合征(PCOS)大鼠胰岛素抵抗的作用。方法:21日龄雌性SD大鼠适应性喂养3 d后,随机分为正常对照组(6只)和模型组(14只)。PCOS模型组将来曲唑(1.0 mg/kg)溶于1%羧甲基纤维素(2 mg/kg)中,每日灌胃,连续21 d,正常对照组则每日给予等量的0.5%羧甲基纤维素,连续21 d,造模成功的大鼠随机分为模型组(6只)、防风通圣丸组(7只),实验干预结束时观察大鼠体质量增长情况、性腺旁脂肪组织重量、卵巢镜下形态学;ELISA法检测大鼠血清睾酮、雌二醇水平;生化法检测空腹血糖、胆固醇、甘油三酯、自由脂肪酸水平;ELISA法检测血清胰岛素水平、计算HOMA-IR指数;葡萄糖刺激胰岛素分泌实验观察胰岛分泌胰岛素情况;Western blotting法检测比目鱼肌胰岛素受体β(IRβ)蛋白水平。结果:与正常对照组相比,模型组大鼠体质量、性腺旁脂肪组织的量显著增加(P<0.05);大鼠卵巢呈多囊样改变;血清睾酮水平显著升高、雌二醇水平显著降低(P<0.05);空腹血糖、血清胰岛素水平、HOMA-IR指数显著升高(P<0.05);血清胆固醇、甘油三酯、自由脂肪酸水平显著升高(P<0.05);葡萄糖刺激胰岛分泌胰岛素显著增加(P<0.05);比目鱼肌IRβ蛋白水平显著降低(P<0.05)。与模型组比较,防风通圣丸组大鼠体质量、性腺旁脂肪组织的量显著降低(P<0.05);空腹血糖、血清胰岛素水平、HOMA-IR指数显著降低(P<0.05);血清胆固醇、甘油三酯、自由脂肪酸水平显著降低(P<0.05);葡萄糖刺激胰岛分泌胰岛素显著降低(P<0.05);比目鱼肌IRβ蛋白水平显著提高(P<0.05)。结论:防风通圣丸改善多囊卵巢综合征大鼠胰岛素抵抗,与提高比目鱼肌IRβ蛋白水平,降低葡萄糖刺激胰岛分泌胰岛素有关。Objective: To explore Fangfeng Tongsheng Pill alleviating insulin resistance(IR) in rats with polycystic ovary syndrome(PCOS). Methods: SD rats were randomly divided into the control group, the model group and the Fangfeng Tongsheng Pill group. Letrozole was used to induce PCOS model. The weight gain, fat content around sexual gland, and morphology under laparoscope were observed after the intervention. Serum levels of testosterone(T) and estradiol(E2) were detected by ELISA;the serum concentrations of fasting blood-glucose, total cholesterol(TC), triglycerides(TGs) and free fatty acid were measured by using biochemical methods. The serum level of insulin was measured by ELISA and the HOMA-IR index was calculated. Insulin secretion of islet was observed with glucose stimulation. The protein level of insulin receptor β(IRβ) in muscle soleus were detected by Western blotting. Results: Compared with those in the control group, the body weight gain and the sexual gland fat were significantly increased(P<0.05);the serum level of T was significantly increased and the serum level of E;was significant decreased(P<0.05);the fasting blood glucose, the serum level of insulin and the HOMA-IR index were increased(P<0.05);the serum levels of TC, TGs and free fatty acid were greatly increased(P<0.05);the insulin secretion of islet under glucose stimulation was significantly increased(P<0.05);the protein level of IRβ in muscle soleus was remarkably decreased in the model group(P<0.05). The ovaries showed polycystic change in the model group. Compared with those in the model group, the body weight gain and the fat around sexual gland were significantly decreased(P<0.05);the fasting blood glucose, the serum level of insulin and the HOMA-IR index were decreased(P<0.05);the serum level of T was significantly decreased and the serum level of E;was significant increased(P<0.05);the serum levels of TC, TGs and free fatty acid were greatly decreased(P<0.05);the insulin secretion of islet under glucose stimulation was significantly de

关 键 词：多囊卵巢综合征 防风通圣丸 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学]