多巴胺D3受体在围绝经期抑郁症发病中的作用  

The role of dopamine D3 receptor in perimenopausal depression

作　　者：曹国芬[1] 朱莉 张苏梅[1] 唐玄兵 CAO Guofen;ZHU Li;ZHANG Sumei;TANG Xuanbing(Xi’an Medical College School of Nursing,Xi’an 710021;College of Forensic Medicine,Xi’an Jiaotong University Health Science Center,Xi’an 710061,China)

机构地区：[1]西安医学院护理学院,陕西西安710021 [2]西安交通大学医学部法医学院,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2022年第3期390-394,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基础研究计划一般项目(No.2020JQ-081)。

摘　　要：目的探究多巴胺D3受体在围绝经期抑郁症发病中的作用。方法基于前期围绝经期抑郁动物模型,采用RT-PCR方法,研究围绝经期抑郁小鼠伏隔核D3受体mRNA的变化;进一步,采用D3受体敲除(D3 receptor knockout,D3RKO)小鼠与相同遗传背景的野生型(wildtype,WT)小鼠,分别构建青年组与围绝经期小鼠,采用强迫游泳、悬尾实验检测小鼠抑郁样行为。结果伏隔核D3受体mRNA在围绝经期明显下降(P<0.05);慢性束缚应激会诱导青年期小鼠D3受体mRNA下降(P<0.05);慢性束缚应激会诱导围绝经期小鼠D3受体进一步下降(P<0.05)。围绝经期D3RKO小鼠相对于围绝经期WT小鼠,在强迫游泳实验中不动时间明显延长(P<0.05);围绝经期D3RKO小鼠相对于青年D3RKO小鼠,在强迫游泳、悬尾实验中不动时间明显延长(P<0.05);围绝经期不会明显改变WT小鼠不动时间(P>0.05)。结论伏隔核多巴胺D3受体在围绝经期明显下降;D3受体的大幅度下降或缺失可能参与围绝经期抑郁症的发病。Objective To investigate the role of dopamine D3 receptor deficiency in depression-like behavior in perimenopausal depression and to explore the role of dopamine D3 receptor in the pathogenesis of perimenopausal depression.Methods Based on the perimenopausal depression animal model,RT-PCR was used to study the changes of D3 receptor mRNA in the nucleus accumbens(NAc)in mice.Furthermore,dopamine D3 receptor knockout(D3RKO)mice and wild type(WT)mice of the same genetic background were used,respectively.Forced swim test(FST)and tail suspension test(TST)were used to assess depressive-like behavior in mice.Results D3 receptor mRNA in the NAc decreased significantly in perimenopause(P<0.05).Chronic immobilization stress(CIS)induced the decrease of D3 receptor mRNA in young mice(P<0.05).CIS induced a further decrease of D3 receptor mRNA in perimenopausal mice(P<0.05).The immobility in the FST was significantly prolonged in perimenopausal D3RKO mice,compared with perimenopausal WT mice(P<0.05).The immobility in both FST and TST was significantly prolonged in perimenopausal D3RKO mice,compared with young D3RKO mice(P<0.05).No significant difference was found between the groups of the WT mice(P>0.05).Conclusion Dopamine D3 receptor in the NAc is significantly decreased during perimenopause.Obvious decrease or deficiency of dopamine D3 receptors may be involved in the regulation of perimenopausal depression.

关 键 词：多巴胺D3受体 围绝经期 抑郁样行为 

分 类 号：R89[医药卫生—法医学]