均三氮苯类除草剂阿特拉津和西玛津及氰草津对PC-12细胞凋亡的影响  

Comparison of PC-12 cells’apoptosis induced by triazine herbicides atrazine,simazine and cyanazine

作　　者：吴昊宇[1] 尹亚萍 吴艳萍[1] 马焜 李百祥[1] WU Haoyu;Yin Yaping;WU Yanping;MA Kun;LI Baixiang(Department of Hygienic Toxicology,Public Health College of Harbin Medical University,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学公共卫生学院毒理学教研室,黑龙江哈尔滨150081

出　　处：《西安交通大学学报（医学版）》2022年第3期462-467,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81973088);中国博士后面上基金资助(No.2019M661310)。

摘　　要：目的探讨均三氮苯类除草剂阿特拉津(ATR)、西玛津(SIM)和氰草津(CYA)致PC-12细胞凋亡及其机制。方法常规培养大鼠肾上腺髓质嗜铬瘤PC-12细胞株,于细胞对数生长期分别以200μmol/L的ATR、SIM和CYA不同分组处理24 h,另设对照组仅添加相同溶剂。采用CCK-8法检测细胞存活率;ROS试剂盒检测PC-12细胞内活性氧(ROS)含量;Real-time PCR和Western blotting法检测Bax、p53、Bcl-2和Caspase-3 mRNA和蛋白表达。结果与对照组相比,ATR组、SIM组和CYA组细胞的存活率均明显下降,细胞内ROS活性均显著升高,Bax、p53和Caspase-3 mRNA及蛋白表达均升高,Bcl-2 mRNA及蛋白表达均明显降低(P<0.01)。与ATR组相比,SIM组和CYA组细胞的存活率均明显上升,细胞内ROS活性均显著下降,p53和Caspase-3 mRNA及蛋白表达均明显降低,Bcl-2 mRNA及蛋白表达均明显升高(P<0.01)。与SIM组相比,CYA组细胞的存活率明显上升,细胞内ROS活性显著下降,Bax、p53和Caspase-3 mRNA及蛋白表达明显降低,Bcl-2 mRNA及蛋白表达明显升高(P<0.01)。结论ATR、SIM和CYA这3种不同的均三氮苯类除草剂均可促进PC-12细胞发生凋亡,其中ATR作用最强,CYA作用最弱。Objective To compare PC-12 cells’apoptosis caused by the serotonin herbicides atrazine(ATR),simazine(SIM)and cyanazine(CYA).Methods The rat adrenal medullary pheochromoma PC-12 cell line was selected for routine culture.At the cell logarithmic growth phase,ATR,SIM and CYA were used at a concentration of 200μmol/L for 24 h,respectively,and the same solvent was added in the control group.The CCK-8 method was used to detect the cell survival rate;the content of reactive oxygen species(ROS)in PC-12 cells was detected;the Real-time PCR and Western blotting methods were used to detect the mRNA and protein expressions of Bax,p53,Bcl-2 and Caspase-3.Results Compared with that in the control group,the survival rate of the cells in ATR group,SIM group and CYA group was significantly decreased.The intracellular ROS activity of the three groups was significantly increased,and the mRNA and protein expressions of Bax,p53 and Caspase-3 were increased.mRNA and protein expressions of Bcl-2 were significantly reduced(P<0.01).Compared with that in ATR group,the cell survival rate of SIM group and CYA group was significantly increased,the intracellular ROS activity of the two groups was significantly decreased,the expressions of Bax,p53 and Caspase-3 mRNA and protein were significantly reduced,and the expressions of Bcl-2 mRNA and protein were significantly increased(P<0.01).Compared with that of SIM group,the cell survival rate of CYA group was significantly increased,while the intracellular ROS activity was significantly decreased;the Bax,p53 and Caspase-3 mRNA and protein expressions were significantly reduced,and Bcl-2 mRNA and protein expressions were significantly increased(P<0.01).Conclusion ATR,SIM and CYA can all promote PC-12 cells’apoptosis;ATR has the strongest effect while CYA has the weakest effect.

关 键 词：阿特拉津 西玛津 氰草津 PC-12细胞 细胞凋亡 

分 类 号：R114[医药卫生—卫生毒理学]