稀土元素钇对体外培养成骨细胞增殖、分化、矿化及凋亡的影响  

The effects of rare earth Yttrium on the proliferation,differentiation,mineralization,and apoptosis of osteoblasts

作　　者：唐春芳 刘喆豪 吴龙火[3] TANG Chun-fang;LIU Zhe-hao;WU Long-huo(Department of Orthopeadics,The First Affiliated Hospital of Gannan Medical University;Gannan Medical University;Pharmacy College,Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院第一附属医院骨科 [2]赣南医学院 [3]赣南医学院药学院,江西赣州341000

出　　处：《赣南医学院学报》2022年第3期221-224,246,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(82060407)。

摘　　要：目的:探讨稀土元素钇对成骨细胞增殖、分化、矿化及凋亡的影响,为研究稀土元素钇对骨质疏松的病理性发展提供一定理论指导。方法:⑴将成骨细胞MC3T3-E1分为对照组(给予PBS)和给药组(给予YCl_(3)至各梯度最终浓度为1×10^(-9),1×10^(-8),1×10^(-7),1×10^(-6),1×10^(-5),1×10^(-4) mol·L^(-1)),利用CCK-8试验检测成骨细胞增殖活力;⑵选取3个浓度梯度YCl_(3)(最终浓度为1×10^(-8),1×10^(-6),1×10^(-4) mol·L^(-1))处理成骨细胞。采用NBT/BCIP试剂盒检测碱性磷酸酶(ALP)表达,根据吸光值分析ALP活性;采用茜素红S染色法检测细胞矿化程度;采用AO/EB双染法测细胞凋亡,利用荧光显微镜观察凋亡细胞形态,分析荧光强度和凋亡情况。结果:稀土元素钇在低剂量(1×10^(-9) mol·L^(-1))时可促进MC3T3-E1细胞增殖,且随着剂量的升高,钇细胞增殖能力活性表现为抑制作用。在浓度为1×10^(-5) mol·L^(-1)时,钇可显著抑制细胞增殖活性。同时,稀土元素钇对细胞ALP活性也表现为低促高抑现象,在浓度为1×10^(-8) mol·L^(-1)时,钇提高了ALP活性,而在浓度高于1×10^(-6) mol·L^(-1)时,ALP活性显著降低。钇显著抑制细胞矿化、诱导细胞凋亡,呈剂量依赖性。结论:稀土元素钇对体外培养MC3T3-E1细胞增殖、分化、矿化及凋亡具有一定的毒性作用,呈剂量依赖性。Objective:To investigate the effects of rare earth Yttrium on the proliferation,differentiation,mineraliza⁃tion,and apoptosis of osteoblasts and to provide some theoretical guidance for studying the effects of Yttrium on the patho⁃logical development of osteoporosis.Methods:⑴Osteoblasts MC3T3-E1 were divided into blank group(PBS)and medication administration group(with YCl_(3) to each gradient,and the final concentration were 1×10^(-9),1×10^(-8),1×10^(-7),1×10^(-6),1×10^(-5),and 1×10^(-4) mol·L^(-1) respectively).CCK-8 assays were used to detect cells proliferation.⑵Three concentra⁃tion gradients YCl_(3)(the final concentration 1×10^(-8),1×10^(-6),1×10^(-4) mol·L^(-1))were selected to treat osteoblasts.The expres⁃sion of alkaline phosphatase(ALP)was detected by NBT/BCIP kit,and ALP activity was analyzed according to absor⁃bance value;Alizarin red S staining was employed to determine the mineralization degree;Apoptosis was detected by AO/EB double staining and observed by fluorescent microscopy,the fluorescent intensity and the apoptosis were analyzed.Results:The rare earth Yttrium at lower concentration(1×10^(-9) mol·L^(-1))promoted the proliferative activity of MC3T3-E1 cells while inhibited it at higher concentration.At the dose of 1×10^(-5) mol·L^(-1),Yttrium could significantly inhibit the proliferative activity.Rare earth element Yttrium also showed a phenomenon of low promotion and high inhibition of ALP activity.Yttrium increased ALP activity at the dose of 1×10^(-4) mol·L^(-1),and decreased it significantly at the dose of more than 1×10^(-6) mol·L^(-1).Yttrium showed inhibitory effects on the mineralization and enhanced cell apoptosis in MC3T3-E1 cells,in a dose dependent manner.Conclusion:The rare earth Yttrium exhibited inhibiting activities in the prolifera⁃tion,differentiation,mineralization,and apoptosis of MC3T3-E1 cells in a dose-dependent manner.

关 键 词：钇 细胞增殖 ALP活性 细胞矿化 骨质疏松 

分 类 号：R992[医药卫生—毒理学]