GC测定蒙药安神补心六味丸中木香烃内酯的含量  

Determination of Costunolide in Mongolian Medicine Anshen Buxin Liuwei pills by GC

作　　者：美丽 荣军 王徽 新图雅 于水 陈红梅[2] MEI Li;RONG Jun;WANG Hui;XIN Tu-ya;YU Shui;CHEN Hong-mei(College of Mongolian Medicine,Inner Mongolia University for Nationalities,Tong Liao 028000,China;Affiliated Hospital of Inner Mongolia University for Nationalities,Tong Liao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学附属医院,内蒙古通辽028000

出　　处：《中国民族医药杂志》2022年第4期27-29,共3页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：国家重点研发项目(蒙医防治优势病种经典方研发平台及质量标准体系建设和示范研究项目)(2018YFC1708200);内蒙古自治区重大专项(2019ZD004);内蒙古自治区人民政府蒙医药标准化项目(2020-﹝MB019﹞)。

摘　　要：目的:建立GC测定蒙药安神补心六味丸中木香烃内酯的含量。方法:采用HP-5MS毛细管柱(30 m×0.32 mm×0.25μm),检测器为FID检测器。进样口温度为315℃,FID检测器温度为335℃。柱温为恒温,温度为156℃,保持13 min,进样方式:分流进样,分流比5∶1,进样量:1μL,载气为氮气,空气流速为300 m L·min^(-1),氢气流速为30 mL·min^(-1),尾吹气流速为25 mL·min^(-1)。结果:GC测定木香烃内酯的线性范围为0.103~0.618μg·μL^(-1)(r=0.9999),在此范围内线性关系良好;平均回收率为97.28%、RSD为1.40%。结论:该方法有效可靠,可有效控制蒙药安神补心六味丸的质量。Objective: To establish a gas chromatography method for the Determination of Costunolide in Mongolian Medicine Anshen Buxin Liuwei pills. Methods: HP-5MS capillary column(30 m ×0.32 mm ×0.25 μm) was used for chromatographic analysis. The detector was FID detector. The inlet temperature was 315 ℃, and the detector temperature was 335 ℃. The initial column temperature was 156 ℃,keep in 13min.The gas was nitrogen. The injection method was split injection with a split ratio of 5 ∶1. The injection volume was 1 μL,and the flow rate of hydrogen was 30 mL·min;, The air flow rate was 300 mL·min;. The tail blowing flow rate was 25 m L·min;. Results: The linear ranges of Costunolide were 0.103~0.618 μg(r=0.9999) and the linear ranges were good in this range. The average recoveries were 97.28%, RSD=1.40%.Conclusions: The method is effective and reliable, and can be used for the content determination of Costunolide in Anshen Buxin Liuwei pills.

关 键 词：气相色谱法 木香烃内酯 蒙药安神补心六味丸 

分 类 号：R291.2[医药卫生—民族医学]