Microengineered platforms for characterizing the contractile function of in vitro cardiac models  

作　　者：Wenkun Dou Manpreet Malhi Qili Zhao Li Wang Zongjie Huang Junhui Law Na Liu Craig A.Simmons Jason T.Maynes Yu Sun 

机构地区：[1]Department of Mechanical and Industrial Engineering,University of Toronto,Toronto,ON M5S 3G8,Canada [2]Program in Molecular Medicine,The Hospital for Sick Children,Toronto,ON M5G 1X8,Canada [3]Department of Biochemistry,University of Toronto,Toronto,ON M5S 1A8,Canada [4]Institute of Robotics and Automatic Information System and the Tianjin Key Laboratory of Intelligent Robotics,Nankai University,Tianjin 300350,China [5]School of Mechanical&Automotive Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China [6]School of Mechatronics Engineering and Automation,Shanghai University,Shanghai 200444,China [7]Institute of Biomedical Engineering,University of Toronto,Toronto,ON M5S 3G9,Canada [8]Translational Biology&Engineering Program,Ted Rogers Centre for Heart Research,Toronto,ON M5G 1M1,Canada [9]Department of Anesthesiology and Pain Medicine,University of Toronto,Toronto,ON M5S 1A8,Canada [10]Department of Anesthesia and Pain Medicine,The Hospital for Sick Children,Toronto,ON M5G 1X8,Canada [11]Department of Electrical and Computer Engineering,University of Toronto,Toronto,ON M5S 3G4,Canada [12]Department of Computer Science,University of Toronto,Toronto,ON M5T 3A1,Canada

出　　处：《Microsystems & Nanoengineering》2022年第1期29-50,共22页微系统与纳米工程（英文）

基　　金：The authors acknowledge the Canadian Institutes of Health Research(CIHR)and the Natural Sciences and Engineering Research Council of Canada(NSERC)for financial support through a Collaborative Health Research Projects(CHRP)grant.W.K.D.acknowledges the Ted Rogers Centre for Heart Research Education Fund for a fellowship;N.L.acknowledges the National Natural Science Foundation of China(NSFC)for financial support under Grant 61933008。

摘　　要：Emerging heart-on-a-chip platforms are promising approaches to establish cardiac cell/tissue models in vitro for research on cardiac physiology,disease modeling and drug cardiotoxicity as well as for therapeutic discovery.Challenges still exist in obtaining the complete capability of in situ sensing to fully evaluate the complex functional properties of cardiac cell/tissue models.Changes to contractile strength(contractility)and beating regularity(rhythm)are particularly important to generate accurate,predictive models.Developing new platforms and technologies to assess the contractile functions of in vitro cardiac models is essential to provide information on cell/tissue physiologies,drug-induced inotropic responses,and the mechanisms of cardiac diseases.In this review,we discuss recent advances in biosensing platforms for the measurement of contractile functions of in vitro cardiac models,including single cardiomyocytes,2D monolayers of cardiomyocytes,and 3D cardiac tissues.The characteristics and performance of current platforms are reviewed in terms of sensing principles,measured parameters,performance,cell sources,cell/tissue model configurations,advantages,and limitations.In addition,we highlight applications of these platforms and relevant discoveries in fundamental investigations,drug testing,and disease modeling.Furthermore,challenges and future outlooks of heart-on-a-chip platforms for in vitro measurement of cardiac functional properties are discussed.

关 键 词：CARDIAC CONTRACTILE REGULARITY 

分 类 号：R318[医药卫生—生物医学工程]