3D打印聚乳酸-羟基乙酸共聚物数字化仿生组织工程骨支架修复兔股骨干长节段骨缺损研究  

3D printed polylactic acid glycolic acid copolymer digital bionic tissue engineering bone scaffold for repairing long segmental bone defects of femoral shaft in rabbits

作　　者：阿力木·克热木 麦麦提艾力·阿布力克木 帕孜拉·艾拉 梁志林[1] 艾克白尔·吐逊[1] Alimu·Keremu;Maimaiti·Aili Ablikmu;Pazila·Aila;Liang Zhilin;Aikebaier·Tuxun(Department of Traumatology,First People’s Hospital of Kashgar,Kashgar 844000,China)

机构地区：[1]喀什地区第一人民医院创伤骨科,844000

出　　处：《中华实验外科杂志》2022年第3期515-518,共4页Chinese Journal of Experimental Surgery

基　　金：新疆维吾尔自治区优秀青年项目(2019Q057)。

摘　　要：目的探讨3D打印聚乳酸-羟基乙酸共聚物(PLGA)数字化仿生组织工程骨支架对兔股骨干长节段骨缺损的修复效果。方法取19只新西兰大白兔,1只用于3D打印PLGA数字化仿生组织工程骨支架制备,18只制备股骨干长节段骨缺损模型,分为自体骨移植组(A组)、传统支架组(B组)、组织工程骨支架组(C组)各6只。在术后4、8、12周做骨愈合观察、测定成骨基因Ⅰ型胶原蛋白(Col-Ⅰ)、骨涎蛋白(BSP)、骨桥蛋白(OPN)、骨钙蛋白(OCN)及骨形态发生蛋白2(BMP-2)/转录因子2(Runx2)通路因子表达。多组间用单因素方差分析,两两比较用LSD-t检验。结果术后4、8、12周C组Lane-Sandhu评分[(5.67±0.85)、(7.29±0.96)、(9.89±1.21)分]、Col-Ⅰ(2.75±0.22、5.01±0.87、7.22±1.25)、BSP(1.64±0.33、3.11±0.43、5.84±0.78)、OPN(2.67±0.43、4.15±0.98、6.98±1.29)、OCN(1.98±0.22、3.42±0.43、5.70±0.98)、BMP-2(2.21±0.43、4.00±0.53、5.11±0.87)、Runx2(3.32±0.34、5.57±0.67、7.98±1.89)高于B、A组,差异有统计学意义(F=22.499、6.635、6.692;21.582、7.917、6.084;8.835、9.398、9.607;9.607、6.185、5.361;9.277、7.256、5.115;6.374、15.320;9.117、10.424;6.042、5.053,P均<0.05)。结论3D打印聚乳酸-羟基乙酸共聚物数字化仿生组织工程骨支架可经促进骨组织生成,修复股骨干长节段骨缺损。Objective To investigate the repair effect of 3D printed polylactic acid-hydroxyacetic acid copolymer(PLGA)digital biomimetic tissue-engineered bone scaffold on long-segment bone defect of rabbit femoral shaft.Methods Totally 19 New Zealand white rabbits,one for 3D printing PLGA digital bionic tissue-engineered bone scaffold preparation,and 18 for long segment bone defect model of femoral shaft,were divided into autologous bone transplantation group(group A),traditional scaffold group(group B)and tissue-engineered bone scaffold group(group C).Bone healing was observed and osteogenic genes typeⅠcollagen(Col-Ⅰ),bone salivary protein(BSP),osteopontin(OPN)and osteocalcin protein(OCN)and bone morphogenesis protein 2(BMP-2)/transcription factor 2(Runx2)pathway factors were detected.One way ANOVA was used for comparison between groups,and LSD-t test was used for pairwise comparison.Results At 4,8 and 12 weeks after operation,Lane Sandhu score(5.67±0.85,7.29±0.96,9.89±1.21),Col-Ⅰ(2.75±0.22,5.01±0.87,7.22±1.25),BSP(1.64±0.33,3.11±0.43,5.84±0.78),OPN(2.67±0.43,4.15±0.98,6.98±1.29),OCN(1.98±0.22,3.42±0.43,5.70±0.98),BMP-2(2.21±0.43,4.00±0.53,5.11±0.87)and Runx2(3.32±0.34,5.57±0.67,7.98±1.89)were significantly higher in group C than those in group B and group A(F=22.499,6.635,6.692;21.582,7.917,6.084;8.835,9.398,9.607,6.185,5.361;9.277,7.256,5.115;6.374,15.320;9.117,10.424;6.042,5.053,all P<0.05).Conclusion 3D printed polylactic acid glycolic acid copolymer digital bionic tissue-engineered bone scaffold can promote bone tissue formation and repair long segmental bone defects of femoral shaft.

关 键 词：3D打印 聚乳酸-羟基乙酸共聚物 骨支架 股骨 骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]