二倍半萜类天然产物的生物合成研究进展  

Research progress on the biosynthesis of natural sesterterpenoids

作　　者：李德森 陈月桂 郭凯[1] 凌伊 刘燕[1,2] 黎胜红[1,2] LI De-sen;CHEN Yue-gui;GUO Kai;LING Yi;LIU Yan;LI Sheng-hong(State Key Laboratory of Southwestern Chinese Medicine Resources,and Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]成都中医药大学省部共建西南特色中药资源国家重点实验室/中医药创新研究院,四川成都611137 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201 [3]中国科学院大学,北京100049

出　　处：《药学学报》2022年第4期931-942,共12页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金重点项目(21937006);国家自然科学基金面上项目(31770340);云南省生物医药重大专项(2019ZF011-2)。

摘　　要：二倍半萜是由五个异戊二烯单元构成、生物合成来源于香叶基法尼基焦磷酸酯的一类珍稀萜类天然产物,迄今仅报道了1 300余个,其分布广泛、结构新颖复杂、生物活性显著。近10年来,随着基因组挖掘和异源表达技术的发展,二倍半萜生物合成研究取得了系列重要进展。本文主要概述了二倍半萜生物合成途径的研究,包括香叶基法尼基焦磷酸酯合酶、二倍半萜合酶和氧化酶的生化功能和催化机制,以期为该类天然产物生物合成、生物活性与合成生物学的深入研究提供参考。Sesterterpenoids, composed of five isoprene units and biosynthetically derived from geranylfarnesyl diphosphate(GFDP), are a class of the precious terpenoids with approximately 1 300 natural products known to date. Natural sesterterpenoids are widely distributed and possess extreme structural complexity and diversity and remarkable biological activity. In recent decades, a series of important progresses have been made in sesterterpenoid biosynthesis with the development of genome mining and heterogeneous expression technologies. This paper mainly focuses on the advances in sesterterpenoid biosynthesis, including the biochemical functions and catalytic mechanisms of GFDP synthases, sesterterpene synthases and oxidases. This review would lay the foundation for in-depth investigation on the biosynthesis, biological activities and synthetic biology of sesterterpenoids.

关 键 词：天然产物 二倍半萜 生物合成 香叶基法尼基焦磷酸酯合酶 二倍半萜合酶 氧化酶 

分 类 号：R284[医药卫生—中药学]