重组酶聚合酶扩增技术原理及其在医学检验中的应用进展  

Principle of recombinase polymerase amplification and its application progress in medical laboratory science

作　　者：尚美云 邓少丽[1] 鲁卫平[1] 黄庆 Shang Meiyun;Deng Shaoli;Lu Weiping;Huang Qing(Department of Clinical Laboratory,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing 400042,China)

机构地区：[1]陆军军医大学(第三军医大学)大坪医院检验科,重庆400042

出　　处：《中华检验医学杂志》2022年第4期423-427,共5页Chinese Journal of Laboratory Medicine

基　　金：新冠肺炎疫情应急科研项目(CWS20C008);生物安全建设项目(A3702022001)。

摘　　要：重组酶聚合酶扩增(RPA)技术是一种新型核酸恒温扩增检测技术。该技术可以在37~42℃条件下实现待测靶标的快速检测。它具有反应灵敏度高、特异性强、对仪器依赖程度低且可整合多种检测模式等优点,特别适用于基层和现场即时检测。本文从RPA技术的基础理论和实验要点出发概述了开展该项研究需要注意的要点,综述了RPA技术在医学检验领域的应用现状和相关问题,并展望了其未来发展的广泛前景。Recombinase polymerase amplification(RPA)is a novel technology for nucleic acid isothermal amplification.It can achieve the rapid amplification and detection of a target gene under 37-42℃.This amplification method is highly sensitive,more specific and less instrument-dependent than other existing methods,and it can also integrate multiple detection modes.Therefore,it is especially suitable for applying in low-resource settings and conducting point-of-care tests.Starting from the reaction principles and the experimental design of RPA,this article pointed out some key points when using RPA in a clinical setting.The current development and related problems of RPA were concluded and the various future uses of this method were also prospected.

关 键 词：实验室技术和方法 核酸扩增技术 恒温扩增 重组酶聚合酶扩增 

分 类 号：R440[医药卫生—诊断学]