水稻高温白化复绿突变体tcd52的遗传分析和分子定位  

Genetic analysis and molecular mapping of high-temperature albino regreen mutant tcd52 in rice

作　　者：李军 朱梦珂 田激旋 陈瑜 林冬枝[1,2] 董彦君[1,2] LI Jun;ZHU Mengke;TIAN Jixuan;CHEN Xu;LIN Dongzhi;DONG Yanjun(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Institute of Genetics,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学遗传研究所,上海200234

出　　处：《上海师范大学学报（自然科学版）》2022年第2期243-250,共8页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(30971552);上海市产业技术体系建设项目[沪农科产字(2021)第3号];上海市科技兴农项目(2021-02-00-12-F00769);上海市科委项目(19391900200)。

摘　　要：粳稻品种“嘉花1号”经甲基磺酸乙酯(EMS)化学诱变处理,获得一个稳定遗传水稻幼苗高温白化复绿突变体tcd52.该突变体在高温(>24℃)条件下,二叶期叶色呈白色失绿,三叶期开始复绿,四叶期后与野生型没有明显差异;而在低温(20℃)条件下,tcd52突变体苗期叶色与野生型一致呈绿色,无白化现象.利用该突变体tcd52与“培矮64S”杂交构建F_(2)遗传群体,发现苗期的高温白化复绿叶色性状受到一对隐性核基因控制,并将该突变基因(tcd52)定位在水稻第5染色体上的ID05M16025和ID05M16113分子标记之间的127 kb区间内,经测序推定突变基因是编码PPR蛋白的基因LOC_Os05g49920.结果表明:tcd52是一个受高温响应且影响水稻早期叶绿体发育的关键基因.今后将进一步对tcd52基因进行研究,以加深了解温度对水稻叶绿体分子发育机理.Japonica rice variety“Jiahua 1”was treated with Ethyl methyl sulfone(EMS)chemical mutagenesis to obtain a stable genetic rice seedling high temperature albino and regreening mutant tcd52.Under high temperature(>24℃),the tcd52 mutant showed white chlorosis at the 2-leaf stage,re-greening at the 3-leaf stage,and no significant difference from the wild type after the 4-leaf stage.By contrast,under low temperature(20℃),the leaf color of the tcd52 mutant at the seedling stage was the same as that of the wild type,and it was green,and there was no albino phenomenon.The F_(2)genetic population was constructed by hybridizing the mutant tcd52 with Peiai 64S.It was found that the high temperature albino regreen leaf color trait at seedling stage was controlled by a pair of recessive nuclear genes.The mutant gene(tcd52)was located in the 127 kb interval between ID05M16025 and ID05M16113 molecular markers on rice chromosome 5.The mutant gene was presumed to be the gene LOC_Os05g49920 encoding PPR protein.Collectively,tcd52 is a key gene that responds to high temperature and affects early rice chloroplast development.In the future,further study of tcd52 gene will help to deepen the understanding of temperature on the molecular development mechanism of rice chloroplast.

关 键 词：水稻 叶色突变 高温敏感 遗传分析 分子定位 

分 类 号：Q344[生物学—遗传学]