肿瘤抗原肽M1-10的抑瘤活性研究  

The Anti-tumor Activities of Tumor Antigen Peptide M1-10

作　　者：黄文强 卜会铜 裴超柱 黄明敏 谭拥军[1] HUANG Wenqiang;BU Huitong;PEI Chaozhu;HUANG Mingmin;TAN Yongjun(College of Biology,Hunan University,Changsha 410082,China)

机构地区：[1]湖南大学生物学院,长沙410082

出　　处：《激光生物学报》2022年第2期121-128,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(81773169);湖南省自然科学基金面上项目(2020JJ4180)。

摘　　要：基于生物信息学分析筛选出一段源于转录因子FOXM1的肿瘤抗原肽M1-10。通过淋巴细胞增殖、干扰素γ释放及细胞杀伤等试验,证实M1-10肽段可在小鼠体内诱导有效的免疫记忆,与HJURP、MELK、VEGFR1、VEGFR2来源的四种抗原肽联用可产生更好的效果。运用小鼠乳腺癌移植瘤模型和MMTV-PyMT原发乳腺癌模型开展M1-10单独或联合四种抗原肽的肿瘤预免疫试验,发现M1-10单独使用可发挥显著的抑瘤效果,联合四种抗原肽的抑瘤效果更强。总之,本研究开发了一种肿瘤抗原肽,该肽免疫原性强,单独或与其他肿瘤抗原肽联用时可实现有效的肿瘤免疫。该结论为FOXM1的抗肿瘤机制提供了进一步的见解。Based on bioinformatics analysis,a transcription factor FOXM1-derived tumor antigen peptide M1-10 was screened out.Through the experiments of lymphocyte proliferation,interferon gamma release,and cell killing,we found that M1-10 peptide could stimulate the immune memory in vivo and produce better effects when combined with HJURP,MELK,VEGFR1,and VEGFR2-derived four antigens.Tumor pre-immunization experiments were carried out with the mouse models of grafted breast cancer and MMTV-PyMT primary breast cancer.Compared with the control group,M1-10 alone produced significant anti-tumor effects,and M1-10 plus four antigen generated stronger anti-tumor effects.In conclusion,the study developed the tumor antigen peptide M1-10 with a strong immunogenicity,which could result in effective tumor immunity when used alone or combined with other tumor antigen peptides.This conclusion provides further insights into the antitumor mechanism of FOXM1.

关 键 词：肿瘤抗原肽M1-10 转录因子FOXM1 免疫记忆 肿瘤免疫 小鼠 

分 类 号：Q730.3[生物学—分子生物学]