哈维氏弧菌感染对红鳍东方鲀IL-6基因DNA甲基化的影响  

Effects of pathogen Vibrio harveyi infection on DNA methylation of IL-6 gene in tiger puffer Takifugu rubripes

作　　者：王晨诗 黄馨笛 崔晓玉 倪萍 叶仕根 王华 高东旭 雷威 WANG Chenshi;HUANG Xindi;CUI Xiaoyu;NI Ping;YE Shigen;WANG Hua;GAO Dongxu;LEI Wei(College of Fisheries and Life Science, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China;National Marine Environmental Monitoring Center, Dalian 116023, China)

机构地区：[1]大连海洋大学水产与生命学院,大连市海珍品疾病防控重点实验室,农业农村部北方海水增养殖重点实验室,辽宁大连116023 [2]国家海洋环境监测中心,辽宁大连116023

出　　处：《大连海洋大学学报》2022年第2期221-226,共6页Journal of Dalian Ocean University

基　　金：辽宁省科技计划博士启动基金(20180540023);国家自然科学基金(41806187,42006126)。

摘　　要：为探讨哈维氏弧菌Vibrio harveyi感染对红鳍东方鲀Takifugu rubripes白细胞介素6(interleukin-6,IL-6)基因DNA甲基化的影响,试验选用体质量为(100.5±10.8)g的红鳍东方鲀,设对照组和感染组(哈维氏弧菌浓度为10^(7) cfu/mL),鱼体在菌液中浸泡12 h后转移到无致病性病原的干净海水中继续饲养7 d,然后进行样品采集,检测红鳍东方鲀脾脏的病理变化、IL-6基因的相对表达量及其启动子区域DNA甲基化变化。结果表明:发病组红鳍东方鲀脾脏有大量坏死灶,未发病组无明显坏死灶;发病组脾脏IL-6 mRNA相对表达量显著高于对照组和未发病组(P<0.05),未发病组和对照组间无显著性差异(P>0.05);未发病组IL-6基因启动子区域DNA甲基化水平明显高于发病组(P<0.05)。研究表明,哈维氏弧菌感染后,红鳍东方鲀表现出不同的抗病能力,且抗病能力不同的红鳍东方鲀在IL-6基因启动子区域DNA甲基化水平存在明显差异。To investigate effect of pathogen Vibrio harveyi infection on DNA methylation of the interleukin-6(IL-6)gene in tiger puffer Takifugu rubripes,tiger puffer with body mass of(100.5±10.8)g was exposed to clean sea water(control group)and V.harveyi(10^(7) cfu/mL)(treatment group)for 12 h and then transferred to clean sea water without pathogens for 7 d when histological changes,and the relative expression level of IL-6,and DNA methylation levels and patterns in the IL-6 promoter region were determined in the spleen of tiger puffer.The results showed that there was a large degree of cell necrosis in the diseased group,and no obvious necrotic areas were found in the non-diseased group.There was significantly higher mRNA level of IL-6 in the spleen in the diseased group than that in the control and non-diseased groups(P<0.05),without significant difference between the control and non-diseased groups(P>0.05).The methylation level of IL-6 was shown to be significantly higher in the spleen of the non-diseased group than that in the diseased group(P<0.05).These findings indicated that tiger puffer chalenged with V.harveyi showed different degrees of disease resistance,which might be involved in significantly different methylation states at the IL-6 promoter.

关 键 词：水产养殖 红鳍东方鲀 哈维氏弧菌 白细胞介素6 DNA甲基化 

分 类 号：S943[农业科学—水产养殖]