锰胁迫下盐肤木锰富集及生理响应特征  

Characteristics of Manganese Accumulation and Physiological Response of Rhus chinensis under Manganese Stress

作　　者：王悟敏 胡佳瑶 刘文胜[1] 曾文斌[1] WANG Wumin;HU Jiayao;LIU Wensheng;ZENG Wenbin(Collage of Life Sciences and Technology, Central-South University of Forestry and Technology, Changsha 410004, China)

机构地区：[1]中南林业科技大学生命科学与技术学院,长沙410004

出　　处：《西北植物学报》2022年第4期628-636,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(42177018);湖南省自然科学基金(2021JJ31147);湖南省教育厅项目(20C1917)。

摘　　要：为揭示盐肤木(Rhus chinensis)锰积累特征与耐受机制,该研究通过盆栽试验方法,分析0(CK)、1、5、10和20 mmol·L^(-1)Mn^(2+)胁迫对半年生盐肤木幼苗生长、生理生化特征及其锰富集特征的影响。结果表明:(1)盐肤木在Mn^(2+)浓度为0~10 mmol·L^(-1)条件下生长发育状况良好,且在5 mmol·L^(-1)Mn^(2+)处理下叶片舒展,叶片颜色较深,生长最佳,而在20 mmol·L^(-1)Mn^(2+)条件下部分叶片出现褐色斑点、萎蔫卷边的现象;随着Mn^(2+)浓度的升高,盐肤木幼苗的生物量呈先升高后下降的趋势,并在5 mmol·L^(-1)Mn^(2+)胁迫时最高。(2)随着Mn^(2+)浓度的升高,盐肤木叶片中光合色素含量呈先升后降的趋势,且在Mn^(2+)浓度为5 mmol·L^(-1)时达到峰值。(3)随着Mn^(2+)浓度的升高,盐肤木幼苗叶片SOD、POD和CAT活性以及可溶性糖、可溶性蛋白、游离脯氨酸含量均呈先增加后下降的趋势,且多在Mn^(2+)浓度为10 mmol·L^(-1)时达到峰值,而其MDA含量随着Mn^(2+)浓度的升高逐渐增加。(4)随着Mn^(2+)浓度的升高,盐肤木地上部和地下部锰含量及总锰含量均逐渐增加,其锰生物富集系数下降且均大于1,其转运系数则呈增加的趋势且均小于1。研究发现,盐肤木具有较强的锰富集、转运能力与锰耐受能力,属于锰根部囤积型耐重金属植物,它主要通过积累渗透调节物质、增强抗氧化酶活性以及在根部囤积锰的方式来应对锰胁迫。In order to reveale the characteristics of manganese accumulation characteristics and tolerance mechanism of Rhus chinensis,this study investigated the effects of Mn^(2+) concentrations of 0(CK),1,5,10 and 20 mmol·L^(-1) on the growth,physiological and biochemical characteristics and manganese accumulation characteristics of R.chinensis seedlings of half a year old by pot experiments.The results showed that:(1)under the conditions of Mn^(2+) concentration of 0-10 mmol·L^(-1),R.chinensis seedlings grew well.When Mn^(2+) concentration attained to 5 mmol·L^(-1),the leaves spread,were dark in color and grew best;while Mn^(2+) concentration attained to 20 mmol·L^(-1),some leaves showed brown spots and wilting and curling.With the increasing of Mn^(2+)concentration,the biomass of R.chinensis seedling increased significantly at first and then decreased,and reached the highest when the concentration of Mn^(2+) was 5 mmol·L^(-1).(2)With the increasing of Mn^(2+) concentration,the photosynthetic pigment content in R.chinensis leaves increased at first and then decreased.When Mn^(2+) concentration attained to 10 mmol·L^(-1),the photosynthetic pigment content in R.chinensis reached the highest.(3)With the increasing of Mn^(2+) concentration,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),and the contents of soluble sugar,soluble protein and free proline of R.chinensis seedling leaves increased at first and then decreased,and most of these values reached the maximum when Mn^(2+) concentration attained to 10 mmol·L^(-1).Malondialdehyde(MDA)content of R.chinensis increased with the rising Mn^(2+) concentration.(4)With the increasing of Mn^(2+) concentration,the manganese content of aboveground and underground part and total manganese content of R.chinensis seedlings gradually increased.The bioconcentration factors(BCF)of R.chinensis decreased significantly with the increase of Mn^(2+) concentration,and all of them were higher than one.The translocation factor(TF)increased significantly,all of

关 键 词：盐肤木 锰胁迫 富集特征 生理生化特征 

分 类 号：Q945.78[生物学—植物学]