枇杷MADS-box全基因组鉴定及其在果实成熟过程中的潜在作用  

Genome-wide identification and analysis of MADS-box gene family in loquat and its potential role in fruit ripening

作　　者：刘楠 寇燕 李小婷[1,2] 陈旭[1,2] 高欢欢 张晶 王超 郑嘉敏 郑国华[1,2] LIU Nan;KOU Yan;LI Xiaoting;CHEN Xu;GAO Huanhuan;ZHANG Jing;WANG Chao;ZHENG Jiamin;ZHENG Guohua(College of Horticulture,Fujian Agriculture and Forestry University;Institute of Storage Science and Technology of Horticultural Products,Fuzhou,Fujian 350002,Chin)

机构地区：[1]福建农林大学园艺学院 [2]福建农林大学园艺产品贮运保鲜研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2022年第3期351-360,共10页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建农林大学科技创新专项(KLH19004A).

摘　　要：利用生物信息学方法,从全基因组水平鉴定枇杷MADS-box基因家族成员,并对其系统进化关系、保守结构域、基因结构、基因复制和启动子顺式作用元件进行分析;同时,结合转录组数据和qRT-PCR结果,分析鉴定出的MADS-box基因家族成员在不同品种和不同成熟阶段的表达情况.本研究共鉴定出89个枇杷MADS-box基因(命名为EjMADS1~EjMADS89),其N端都含有MADS结构域.系统发育树显示,Ⅰ型MADS-box基因有31个,Ⅱ型有58个.Ⅱ型MADS-box基因可划分为14个亚族,同属于一个亚家族的基因有着相似的保守基序.MADS-box基因以不同密度分布在第1~17号染色体上,存在12对串联重复基因.转录组分析表明,在果实成熟过程中,有8个MADS-box基因在果实成熟期间差异表达,其中,EjMADS26、EjMADS43和EjMADS41这3个基因序列分别与其他调控果实成熟的SEP亚族基因高度相似,且它们的启动子中都含有赤霉素和脱落酸响应元件.另外,EjMADS37和EjMADS05在不同品种间(特早熟/中晚熟)有明显表达差异.综上,推测EjMADS26、EjMADS43、EjMADS41、EjMADS37和EjMADS05对果实成熟有一定的调控作用.The members of MADS-box gene family in loquat(Eriobotrya japonica Lindl.) were identified at the genome-wide level by bioinformatics method, and their phylogenetic relationship, conserved domain, gene structure, gene replication and promoter cis-acting elements were analyzed. Based on the transcriptome data and qRT-PCR results, the expression patterns of MADS-box gene family members in different varieties were further interpreted at different stages of maturity. A total of 89 MADS-box genes were identified, all of which contained MADS domain at the N-terminal. Phylogenetic tree demonstrated that 31 EjMADS-box were classified into type Ⅰ and the other 58 EjMADS-box genes belonged to type Ⅱ. The EjMADS-box genes of type Ⅱ can be divided into 14 subfamilies, and shared similar conserved motifs. The EjMADS-box genes were distributed on chromosome 1-17, and comprised 12 pairs of tandem repeats. Transcriptomic analysis showed that 8 MADS-box genes were differentially expressed during fruit ripening, among which EjMADS26, EjMADS43 and EjMADS41 were highly similar to other SEP genes that regulated fruit ripening, and their promoters all contained gibberellic acid and abscisic acid response elements. In addition, EjMADS37(subfamily of SVP) and EjMADS05(subfamily of AGL15) were significantly different in different varieties(especially early-/late-ripening). Therefore, it can be speculated that EjMADS26, EjMADS43, EjMADS41, EjMADS37 and EjMADS05 could regulate the ripening of loquat fruit to some extent.

关 键 词：枇杷 全基因组 MADS-BOX基因 果实成熟 

分 类 号：S667.3[农业科学—果树学]