不同前处理对测定甜高粱茎秆汁液3种糖含量的影响  

Determination of three soluble sugars in stem juice of sweet sorghum by anthrone colorimetry

作　　者：周振祥 赵博 王唯先 仝骁鹏 郑剑波 李艳红 高建明[1] 罗峰[1] 孙守钧[1] 裴忠有[1] ZHOU Zhenxiang;ZHAO Bo;WANG Weixian;TONG Xiaopeng;ZHENG Jianbo;LI Yanhong;GAO Jianming;LUO Feng;SUN Shoujun;PEI Zhongyou(Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院,天津300384

出　　处：《草业科学》2022年第5期940-948,共9页Pratacultural Science

基　　金：“十三五”国家重点研发计划“七大农作物育种”专项(2017YFD0100500);国家公益性行业(农业)科研专项(201503134)。

摘　　要：茎秆含糖量是甜高粱(Sorghum dochna)重要的农艺性状,其可溶性糖的组分与含量的测定,是甜高粱QTL定位和性状改良的前提。本研究以粒用高粱的‘忻粱52’与甜高粱‘W452’重组自交系F8代255个群体为材料,采用改良蒽酮比色法来测定蔗糖、果糖和葡萄糖含量。结果表明:可溶性总糖的最佳显色时间为10 min,显色温度为100℃;测定蔗糖的最佳处理条件为1 mol·L^(−1)的KOH溶液处理10 min;果糖测定的最佳显色温度为50℃,显色时间为3 min。优化过后的蒽酮比色法能测得较稳定的数值,精密度和稳定性较好,可用于测定甜高粱汁液的糖含量。重组自交系后代糖锤度与可溶性糖总量之间呈极显著正相关关系(P<0.01),相关系数为0.885,线性回归方程为y=37.082×可溶性糖总量+3.990。Sugar content in stem is an important agronomic character of sweet sorghum(Sorghum dochna).The determination of the composition and content of soluble sugar in sweet sorghum is the premise of QTL mapping and character improvement of sweet sorghum.In this study,the content of sucrose,fructose and glucose was determined by improved anthrone colorimetry using 255 populations of‘Xinliang 52’and‘W452’recombinant inbred Lines of grain sorghum as materials.The results showed that the best color developing time of soluble sugar was 10 min and the color developing temperature was 100℃.The optimal treatment condition for sucrose determination was 1 mol·L^(−1) KOH solution for 10 min.The optimum temperature and time for the determination of fructose were 50℃and 3 min.The optimized anthrone colorimetric method can be used to determine the sugar content of sweet sorghum juice with good precision and stability.The results showed that there was a significant positive correlation between the weight of the progeny of this variety and the total amount of soluble sugar,and the value of the correlation was 0.885.The Linear regression equation was y=37.082×total soluble sugar+3.990.

关 键 词：甜高粱 可溶性糖 蔗糖 果糖 葡萄糖 锤度 蒽酮比色法 

分 类 号：S566.5[农业科学—作物学]