黄河鲤生长性状的转录组解析  

Transcriptome analysis for growth traits of Cyprinus carpio

作　　者：李佩珍 许建[2] 朱优秀 冯建新 江炎亮 张瀚元[2] LI Peizhen;XU Jian;ZHU Youxiu;FENG Jianxin;JIANG Yanliang;ZHANG Hanyuan(National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, China;Henan Academy of Fishery Sciences, Zhengzhou 450044, China)

机构地区：[1]上海海洋大学,水产科学国家级实验教学示范中心,上海201306 [2]中国水产科学研究院,农业农村部水生动物基因组学重点实验室,北京100141 [3]河南省水产科学研究院,河南郑州450044

出　　处：《中国渔业质量与标准》2022年第2期22-31,共10页Chinese Fishery Quality and Standards

基　　金：国家重点研发计划(2018YFD0900102);中国水产科学研究院基本科研业务费项目(2020B003,2020TD24);河南省现代农业产业技术体系专项(S2014-10)。

摘　　要：通过对同一实验条件下黄河鲤(Cyprinus carpio)群体中体重极大和极小的样本进行转录组测序,挖掘生长相关的基因和信号通路。基于转录组数据进行差异表达基因分析,在脑、肝和肌肉组织中分别筛选出差异表达基因6519个、499个和19个。其中,脑组织中上调基因3443个,下调基因2942个;肝组织中上调基因275个,下调基因186个;肌肉组织中上调基因6个,下调基因5个。对差异表达基因进行了功能注释和通路富集分析,定位到MAPK信号通路、胰岛素(insulin)信号转导通路、Wnt信号通路、ErbB信号通路、肌动蛋白细胞骨架调节等与细胞生长、分化、代谢相关的信号通路。为了验证转录组结果的可靠性,本研究通过荧光定量PCR验证了黄河鲤脑组织中与生长密切相关的13个差异表达基因,其中mapk1、mapk7、mapk10、mapk12、wnt16、wnt8b、wnt10b显著上调(P<0.05),fgf13、ednra、notch2、tgfbr2、fgf19、lpl显著下调(P<0.05)。本研究通过组学数据分析和实验验证筛选了生长相关的基因,为深入挖掘黄河鲤生长性状调控机制和分子标记辅助育种提供了研究依据。The transcriptome sequencing of Cyprinus carpio samples with maximum and minimum body weight under the same experimental conditions were performed to explore growth-related genes and signaling pathways.Differentially expressed genes(DEGs)were analyzed based on transcriptome data,and DEGs were screened out from brain with 6519,liver with 499 and muscle with 19,respectively.The numbers of up-regulated genes in brain,liver and muscle tissues were 3443,275 and 6,while down-regulated genes in brain,liver and muscle tissues were 2942,186 and 5.Functional annotation and pathway enrichment analysis were carried out for DEGs,and signaling pathways related to cell growth,differentiation and metabolism were found,including MAPK signaling pathway,insulin signaling pathway,Wnt signaling pathway,ErbB signaling pathway and actin cytoskeleton regulation,etc.In order to verify the reliability of transcriptome results,13 DEGs closely related to growth in the brain tissue of Cyprinus carpio were verified by fluorescence quantitative polymerase chain reaction(PCR),among which mapk1,mapk7,mapk10,mapk12,wnt16,wnt8b and wnt10b were significantly up-regulated genes(P<0.05),and fgf13,ednra,notch2,tgfbr2,fgf19 and lpl were significantly down-regulated genes(P<0.05).In this study,growth-related genes were screened by omics data analysis and experimental validation to provide the research basis for further exploring the regulation mechanism of growth traits and molecular marker-assisted breeding of Cyprinus carpio.

关 键 词：鲤 生长性状 转录组分析 差异表达基因 信号通路 

分 类 号：S917[农业科学—水产科学]