手性向列型介孔碳固定漆酶电极用于检测邻苯二酚  

Enzyme electrode of laccase immobilized on chiral nematic mesoporous carbon for determination of pyrocatechol

作　　者：张锋[1] 阿茹拉 李斌[1] ZHANG Feng;A Ru-la;LI Bin(College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China)

机构地区：[1]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040

出　　处：《陕西科技大学学报》2022年第3期100-105,共6页Journal of Shaanxi University of Science & Technology

基　　金：中央高校基本科研业务费专项资金项目(2572018BC17);黑龙江省大学生创新创业训练计划项目(201910225242)。

摘　　要：采用纤维素纳米晶(NCC)为模板,正硅酸甲酯为硅源前驱体,蒸发自组装、高温碳化制备了手性向列型介孔碳.SEM和BET比表面积及孔隙分析表明该介孔碳材料保持了NCC高度有序的左旋手性向列型微观结构,比表面积高达449 m^(2)g^(-1),孔体积为0.51 cm^(3)g^(-1),平均孔径5.43 nm.以手性向列型介孔碳为载体固定漆酶制备漆酶电极,研究了该漆酶电极对邻苯二酚的直接电化学行为和催化氧化还原性能,并成功应用于邻苯二酚的定量检测.结果表明,邻苯二酚在漆酶电极表面能进行直接的电化学反应,该漆酶电极构成的生物传感器对邻苯二酚具有良好稳定的电流响应,线性检测范围为1.1~22.8μmol/L,选择灵敏度为0.016 A/(mol/L),检测限为0.217μmol/L.该漆酶电极具有良好的选择性、稳定性和重现性,较高的灵敏度和较低的检测限,在废水邻苯二酚检测领域具有广阔的应用前景.Chiral nematic mesoporous carbon was prepared by evaporation induced self-assembly and high temperature carbonization of nanocrystalline cellulose(NCC)template with tetramethoxysilane precursor.The SEM and BET specific surface area and porosity analysis indicated that the mesoporous carbon material had retained left-handed chiral nematic structures of NCC.The maximum BET surface area of the mesoporous carbon material was 449 m^(2)g^(-1).The total pore volume and average pore diameter were 0.51 cm^(3)g^(-1) and 5.43 nm,respectively.On this basis,a novel enzyme electrode was fabricated via laccase immobilized on as-prepared mesoporous carbon material,the electrochemical behavior and performance of electro-catalytic oxidation-reduction for pyrocatechol were investigated.And the immobilized laccase electrode biosensor was successfully used for determination of pyrocatechol.The results suggest that the direct electrochemical reaction of pyrocatechol take place at the surface of enzyme electrode.The enzyme electrode biosensor had good and stable current response on pyrocatechol,and the linear detection for pyrocatechol ranged from 1.1 to 22.8μmol/L with a selective sensitivity of 0.016 A/(mol/L)and a detect limit of 0.217μmol/L.The biosensor exhibited excellent selectivity,stability and reproducibility,as well as better sensitivity and a low limit of detection.Therefore,it has great potential for determination of pyrocatechol in industrial wastewater.

关 键 词：介孔碳 漆酶 酶固定 酶电极 邻苯二酚 

分 类 号：TQ150[化学工程—电化学工业] TB34[一般工业技术—材料科学与工程]