高效液相色谱法测定小活络丸中葡萄糖、果糖、蔗糖、麦芽糖  

Determination of glucose,fructose,sucrose and maltose in Xiaohuoluo pills by high performance liquid chromatography

作　　者：刘艳平 朱山 王曾 曾庆真 LIU Yanping;ZHU Shan;WANG Zeng;ZENG Qingzhen(Heze Institute for Food and Drug Control,Heze 274000,China;Heze Economic Development Zone comprehensive supervision department,Heze 274000,China)

机构地区：[1]菏泽市食品药品检验检测研究院,山东菏泽274000 [2]菏泽经济开发区综合监管部,山东菏泽274000

出　　处：《化学分析计量》2022年第5期39-43,共5页Chemical Analysis And Meterage

摘　　要：建立高效液相色谱法同时测定小活络丸中葡萄糖、果糖、蔗糖、麦芽糖含量的方法。以50%乙腈–水溶液作为提取溶剂,进样体积为20μL,以乙腈–水(体积比为75∶25)为流动相,流量为1.0 mL/min,采用ZORBAX Carbohydrate型色谱柱(250 mm×4.6 mm,5μm)进行分离,柱温为40℃;采用示差折光检测器检测,温度为40℃。果糖、葡萄糖、蔗糖、麦芽糖在各自的质量浓度范围内与色谱峰面积线性关系良好,相关系数为0.9992~0.9997,检出限与定量限分别为0.034~0.083、0.067~0.21 mg/mL。平均加标回收率为87.2%~106%,测定结果的相对标准偏差为1.5%~5.0%(n=6)。该方法操作简单,可用于小活络丸中葡萄糖、果糖、蔗糖、麦芽糖的测定,并对小活络丸的质量进行控制。A high performance liquid chromatography(HPLC)method was established for the simultaneous determination of glucose,fructose,sucrose and maltose in Xiaohuoluo pills.Extracted with 50%acetonitrile-aqueoussolution,and the injection volume was 20μL.Acetonitrile water(volume ratio 75∶25)was used as the mobile phase,the flow rate was 1.0 mL/min,and Zorbax carbohydrate column(250 mm×4.6 mm,5μm)was used for separation,the column temperature was 40℃.Differential refraction detector was used for detection,and the temperature was 40℃.The correlation coefficient was 0.9992-0.9997,the detection limit and quantitative limit were 0.034-0.083 and 0.067-0.21 mg/mL,respectively.The average recovery was 87.2%-106%,and the relative standard deviation of the determination results was 1.5%-5.0%(n=6).The method is simple and can be used for the determination of glucose,fructose,sucrose and maltose in Xiaohuoluo pills,and the quality of Xiaohuoluo pills can be controlled.

关 键 词：小活络丸 辅料 高效液相色谱法 

分 类 号：O657.3[理学—分析化学]