63个小麦高代品系的叶锈病抗性鉴定  

Identification of Leaf Rust Resistance in 63 Wheat Advanced Lines

作　　者：董瑞 王佳荣 张静[1] 高璞 张培培 李在峰[1] 刘大群[1] DONG Rui;WANG Jia-rong;ZHANG Jing;GAO Pu;ZHANG Pei-pei;LI Zai-feng;LIU Da-qun(College of Plant Protection,Agticultural University of Hebei,Baoding 071001)

机构地区：[1]河北农业大学植物保护学院,保定071001

出　　处：《植物遗传资源学报》2022年第3期678-690,共13页Journal of Plant Genetic Resources

基　　金：河北省高等学校科学技术研究项目(ZD2021053)。

摘　　要：小麦叶锈病严重危害我国小麦生产安全,培育持久性抗病品种是防治小麦叶锈病最为经济有效的方法之一。本试验通过已知抗病基因连锁的分子标记检测结合基因推导、系谱分析和成株期抗病鉴定对63个小麦高代品系进行抗叶锈病鉴定,进而推导其所含的抗病基因。在苗期,对63个小麦高代品系材料和36个含有已知抗病基因的载体品种,比较了接种17个具有不同毒性的叶锈菌生理小种的抗病反应,并利用10个已知抗叶锈病基因连锁分子标记检测供试材料。2018-2019年度分别在河南周口和河北保定对63份小麦高代品系进行病害严重度调查,筛选具有成株抗叶锈病的品系。结果表明,在63个品系中,含有Lr26、Lr45、Lr17和Lr1的品系分别有18个、14个、11个和10个。此外,含有Lr10、Lr11和Lr46的品系分别有2个,含有Lr2a、Lr2b、Lr15和Lr37的品系各有1个。17个品系在田间表现为成株期慢叶锈病抗性。上述含有抗叶锈病基因的品系为抗叶锈病品种改良提供了重要的育种材料。Wheat Leaf rust seriously distablizes the wheat production in China.Breeding wheat cultivars with durable resistance is considered as one of the most economical and effective methods in disease control.In this study,the Leaf rust resistance in 63 advanced wheat lines were analyzed by combining marker-assisted identification of known resistance genes,pedigree analysis and the field test upon Leaf rust infection.These genotypes together with 36 donor lines(carrying known Leaf rust resistance genes)were inoculated with 17 Puccinia triticina(Pt)races with specific virulence at the seedling stage,and also genotyed using 10 molecular markers associating with known resistance genes.Moreover,these 63 wheat advanced lines were evaluated for Leaf rust severity in Zhoukou city of Henan province and Baoding of Hebei province in the crop season(2018-2019),followed by analyzing variance and calculating the LSD value to identify wheat lines that showed adult plant resistance.Out of 63 lines,18 lines were detected with Lr26,14 harboring Lr45,11 harboring Lr17,as well as 10 lines harboring Lr1.Any of three genes Lr10,Lr11,and Lr46 was found in 2 lines,while either of Lr2a,Lr2b,Lr15 and Lr37 was present in one line.Interestingly,a total of 17 lines showed slow rusting resistance at adult plant stage.Altogether,this study identified many wheat advanced breeding lines containing Leaf rust resistance genes,thus valuable in genetic improvement of wheat cultivars showing Leaf rust resistance.

关 键 词：小麦 叶锈病 抗病基因 基因推导 分子标记 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]