广东湛江蔗区甘蔗白条病的分子检测与鉴定  

Molecular Detection and Identification of Xanthomonas albilineans Causing Leaf Scald of Sugarcane in Zhanjiang,Guangdong Province of China

作　　者：田夏红 赵建英 傅华英[2] 施扬 苏俊波[3] 刘建荣 TIAN Xia-hong;ZHAO Jian-ying;FU Hua-ying;SHI Yang;SU Jun-bo;LIU Jian-rong(Scientific Research Institute of Zhanjiang State Farms,Zhanjiang 524086;National Engineering Research Center forSugarcane,Fujian Agriculture and Forestry University,Fuzhou 350002;South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Science,Guangdong Zhanjiang 524091)

机构地区：[1]广东省湛江农垦科学研究所,湛江524086 [2]福建农林大学国家甘蔗工程技术研究中心,福州350002 [3]中国热带农业科学院南亚热带作物研究所,广东湛江524091

出　　处：《植物遗传资源学报》2022年第3期731-737,共7页Journal of Plant Genetic Resources

基　　金：国家现代农业产业技术体系(糖料)建设专项(CARS-170302、CARS-170708)。

摘　　要：为明确粤西蔗区甘蔗白条病的病原类型,对27份新引进的甘蔗品种进行田间病害调查,并对15份疑似发病植株的蔗茎样品进行病菌分离和PCR检测鉴定。2019年和2020年田间调查结果显示,种植的11个甘蔗品种出现典型的白条病症状,其中桂糖08-1589、德蔗12-88、福农11-2907和福农09-7111在新植蔗和宿根蔗上均有发生,自然发病率为0.2%~9.4%。利用白条黄单胞菌Xanthomonas albilineans特异性引物XAF1/XAR1,对15份病样分离出来的病原细菌进行PCR检测,均可获得608 bp的特异性扩增片段,所得序列完全一致,与来自中国福建、美国佛罗里达州和法国瓜德罗普岛的白条黄单胞菌一致性为100%,在系统进化树中处于同一分支,属于PFGE-B型基因型。本研究证实湛江地区11个甘蔗品种发生的白条病是由白条黄单胞菌引起。To clarify the bacterial pathogen causing sugarcane leaf scald in west of Guangdong province,we conducted field surveys of leaf scald regarding 27 newly-introduced sugarcane varieties in two calendar years(2019 and 2020).For 15 sugarcane stalk samples of suspected diseased plants,isolating the pathogen(s)and molecular identification by PCR were further performed.Based on the results of field trials,eleven sugarcane varieties were found with the typical symptom of leaf scald.Among them,the diseased plants from four varieties GT08-1589,DZ12-88,FN11-2907,and FN09-7111 were found in both the plant cane and ratoon cane,with incidence rates from 0.2%to 9.4%.The bacteria isolated from 15 diseased stalks were detected by PCR with the specific primers XAF1/XAR1 of Xanthomonas albilineans.These fragments are 608 bp in length and share 100%sequence identity to the published nucleotide sequences of X.albilineans isolates in China,USA,and France.These isolates were clustered in the same clade(PFGE-B).Altogether,our results confirmed that leaf scald occurred in 11 cultivars from Zhanjiang sugarcane-planting region was caused by X.albilineans.

关 键 词：甘蔗 白条病 田间症状 分子检测 序列分析 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]