大豆DELLA基因单倍型与花期关联分析及基因编辑靶点鉴定  

Association Analysis of Soybean DELLA Gene Haplotypes with Flowering Time and Identification of Their Gene-editing Target Sites

作　　者：贺米兰 李海洋[1] 黄泽荣 孔凡江 赵晓晖 HE Mi-lan;LI Hai-yang;HUANG Ze-rong;KONG Fan-jiang;ZHAO Xiao-hui(School of Life Sciences,Guangzhou University,Guangzhou 510006)

机构地区：[1]广州大学生命科学学院,广州510006

出　　处：《植物遗传资源学报》2022年第3期787-799,共13页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(32072013)。

摘　　要：DELLA蛋白作为赤霉素负调控因子参与植物开花期调控。与拟南芥DELLA蛋白同源比对,研究发现大豆具有8个DELLA基因,其中GmGAI3a只有GRAS结构域,其他7个DELLA蛋白均具有DELLA结构域和GRAS结构域。对8个大豆DELLA基因在424份材料的自然群体中的单倍型与开花期进行关联分析,发现DELLA基因早花型自然变异已经应用于我国中高纬度地区,推测大豆DELLA基因负向调控开花。利用CRISPR/Cas9技术,在大豆发根系统中验证靶点的编辑效率,成功鉴定出7个DELLA基因的有效靶点,为进一步获得稳定遗传转化的大豆DELLA突变体及解析基因功能提供了重要的靶点信息与理论基础。DELLA proteins are known as negative regulators of gibberellin involved in plant flowering.Eight DELLA genes were identified in soybean genome by sequence alignment with Arabidopsis thaliana(L.)Heynh.DELLA orthologs GmGAI3a has only one GRAS domain,and the other seven DELLA proteins have both DELLA domain and GRAS domain.By performing gene-based association analysis of flowering time in natural population,soybean DELLA haplotypes associating with early-flowering have been detected in soybean accessions collected from the middle and high latitude of China,speculating DELLA genes as negative factors in regulating flowering.CRISPR/Cas9-based editing in soybean hairy root system revealed the editing efficiency at the target sites.Identification of the CRISPR/Cas9 targets of seven DELLA genes provided references for generating stable transgenic DELLA mutants for deciphering their biological functions.

关 键 词：大豆 DELLA 单倍型 花期关联分析 CRISPR/Cas9 

分 类 号：S565.1[农业科学—作物学]