陆地棉早熟相关性状的QTL定位与相关基因的初步鉴定  

QTLs Mapping for Early-maturity Related Traits and Preliminary Identification of Related Genes in Upland Cotton

作　　者：吕婉玉 高阳 潘奥 周娟 杜召海 袁阳 陈煜 王芙蓉[1,2] 张军 LYU Wan-yu;GAO Yang;PAN Ao;ZHOU Juan;DU Zhao-hai;YUAN Yang;CHEN Yu;WANG Fu-rong;ZHANG Jun(College of Life Science,Shandong Normal University,Jinan 250014;Institute of Industrial Crops,Shandong Academy of Agricultural Sciences,Jinan 250100)

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省农科院经济作物研究所,济南250100

出　　处：《植物遗传资源学报》2022年第3期800-810,共11页Journal of Plant Genetic Resources

基　　金：国家现代农业产业技术体系(CARS-15-05);山东省农业良种工程(2020LZGC002)。

摘　　要：早熟性状是棉花遗传改良的目标性状之一,鉴定棉花早熟性状QTL具有重要育种价值。以陆地棉早熟品系EM019(Early-maturity 019)和陆地棉晚熟品种鲁棉研37号(LMY37,Lumianyan 37)为亲本杂交,构建了包含312个株系的重组自交系群体(RILs,recombinant inbred lines),采用复合区间作图法对该RIL群体在山东临清(2019,2020,2021年)、新疆轮台(2019年)4个环境条件下的开花时间、吐絮率、铃重、衣分进行QTL检测,共获得21个QTLs。其中7个与开花时间相关,加性效应在0.30~1.01之间,解释表型变异率为2.56%~17.71%;8个与吐絮率相关,加性效应在-5.81~-2.39之间,解释的表型变异率为4.66%~20.44%。这些QTLs大多成簇分布在A05、D03和D08染色体上。对D08染色体上的一个QTL簇进行相关基因鉴定,筛选出124个相关基因,结合LMY37和EM019在长日照和短日照处理下不同叶龄期的转录组数据,通过GO富集和热图分析,获得了3个可能与早熟相关的基因:GH_D08G0636、GH_D08G0684和GH_D08G0948。本研究为后续棉花早熟相关性状QTL的精细定位和相关基因鉴定奠定了理论基础。Early maturity is one of the target traits in upland cotton(Gossypium hirsutum L.)breeding,and identification of QTL related to early-maturity is of practical value.In our study,we crossed early-maturity 019 line(EM019)with a late-maturing cultivar Lumianyan 37(LMY37),followed by continuous self-pollination and construction of a recombinant inbred line(RIL)population composed of 312 lines.The RIL population was planted in Linqing(2019,2020 and 2021)and Xinjiang(2019).We used composite interval mapping(CIM)method to detect quantitative trait locus(QTLs)underlying the flowering time(FT),percentage of open bolls(POB),boll weight(BW)and lint percentage(LP).A total of twenty-one early-maturity QTL had been identified,and seven QTL were detected with the additive effect ranging from 0.3 to 1.01,explaining 2.56%~17.71%of the phenotypic variation.Eight QTL associating with POB were identified with the additive effect of 5.81~-2.39,explaining 4.66%~20.44%of the phenotypic variation.These QTL were mainly distributed on Chromosomes A05,D03 andD08.A QTL cluster was found on Chromosome D08,in which 124 candidate genes were annotated based on the transcriptome data of LMY37 and EM019 at different true leaf stages under long and short day treatments.Three strong related genes namely GH_D08G0636,GH_D08G0684 and GH_D08G0948,have been identified.Altogether,these QTL identified from this study provided insight for future deciphering the molecular mechanisms of early-maturity related traits in cotton.

关 键 词：棉花 开花时间 吐絮率 铃重 衣分 QTL定位 

分 类 号：S562[农业科学—作物学]