小麦SAPs家族分析及TaSAP1;1耐盐和低磷胁迫功能研究  

Genomic Analysis of Stress Associated Proteins in Wheat and Functional Study of TaSAP1;1 in Salt and Low-Pi Tolerance

作　　者：苏晓帅 张宝华 刘佳静 陈耀仙 田晓 肖凯[2] 李小娟 SU Xiao-shuai;ZHANG Bao-hua;LIU Jia-jing;CHEN Yao-xian;TIAN Xiao;XIAO Kai;LI Xiao-juan(College of Life Sciences,Hebei Agricultural University/Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Baoding 071001;College of Agronomy,Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071001)

机构地区：[1]河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室,保定071001 [2]河北农业大学农学院/华北作物改良与调控国家重点实验室,保定071001

出　　处：《植物遗传资源学报》2022年第3期857-870,共14页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(32071935,31872869);河北省自然科学基金(C2015204057)。

摘　　要：胁迫相关蛋白(SAPs,stress-associated proteins)在植株应答非生物逆境中扮演重要角色,但对小麦中该家族基因信息及成员的功能了解尚少。本研究对小麦SAP家族基因鉴定得到62个成员,并结合系统进化、基因结构和蛋白基序组成进行了分析。应用qRT-PCR研究前期发现的该家族低磷胁迫应答成员TaSAP1;1的表达特征,结果表明其表达量在两种胁迫后均显著增加。该基因存在生物多样性,亚细胞定位分析发现TaSAP1;1定位于细胞核。构建表达载体并转化烟草,获得过表达转基因系(OE,overexpression lines),结合表型特征和植株鲜重、叶面积以及叶绿素含量等参数分析发现,过表达该基因不仅提高了植株耐盐性,还增强了对低磷胁迫的抵御。其中过表达株系较野生型耐盐性和耐低磷能力增加可能分别与部分ABA信号途径基因NtSAPKs表达量增加引起的气孔关闭速度加快和部分磷转运蛋白(PTs,Phosphate transporters)基因的上调表达有关。此外,酵母双杂交技术分析发现TaSAP1;1与小麦转氨酶TaAMTR存在的相互作用可能也参与了该基因抵御胁迫的功能。研究结果丰富了对小麦SAP基因家族的认识,也对其成员参与非生物胁迫的功能有了新理解,为作物综合抗逆遗传改良提供了理论依据。Stress associated proteins(SAPs)play an important role in plant response to abiotic stress,but there was little information about the SAP family and function of its member in wheat.In this study,62 SAP genes were identified from wheat,and analyses of these members were conducted combined phylogenetic tree,gene structure and constitutive motifs.The expression pattern of TaSAP1;1,a member belongs to SAP family and responding to low-Pi stress in our previous study was investigated by qRT-PCR,and the results showed its expression increased after both stresses.Biodiversity analysis revealed that there was diversity of TaSAP1;1 gene and subcellular localization analysis showed that TaSAP1;1 targeted onto nucleus.The expression vector was constructed and transformed into tobacco to obtain the overexpression lines(OE).Combined phenotypic analysis and parameters comparison such as fresh weight,leaf area and chlorophyll content in plants of OE lines and wild-type(WT),it was showed that overexpression of TaSAP1;1 in tobacco not only enhanced the tolerance of seedlings to salt stress,but also to low-Pi stress.The improved tolerance may be related to the increased stomatal closure rate resulting from up-regulation of some ABA signal pathway related genes(NtSAPKs),and the increased expression of some phosphate transporters(PTS)genes,respectively.In addition,the interaction between TaSAP1;1 and a transaminase named TaAMTR detected by yeast-two hybridization assay may also be involved in the process of stress resistance.The results not only enrich the understanding of wheat SAP family,but also have a new insight on the function of its members involved in abiotic stress,which provides a theoretical basis for the genetic improvement of crop comprehensive stress resistance.

关 键 词：小麦 胁迫相关蛋白 盐胁迫 低磷胁迫 功能分析 

分 类 号：S512.1[农业科学—作物学]