连翘提取物对急性酒精中毒大鼠的干预作用及对TIMP-1、HIF-1α水平的影响  

Effects of Fructus Forsythia Extract on the Levels of TIMP-1 and HIF-1αin Acute Alcoholism Rats

作　　者：刘飞[1] 陈妍[2] Liu Fei;Chen Yan(Department of Pharmacy,Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Urumqi 830000,China;Department of Pharmacy,The second Affiliated Hospital of Xinjiang Medical University,Urumqi 830061,China)

机构地区：[1]新疆医科大学附属中医医院(新疆维吾尔自治区中医医院),乌鲁木齐830000 [2]新疆医科大学第二附属医院,乌鲁木齐830061

出　　处：《世界科学技术-中医药现代化》2021年第12期4483-4489,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：新疆维吾尔自治区科学技术厅自然科学基金联合基金(2021D01C369):新疆地方药欧芹根总黄酮对脂联素蛋白表达和多聚化的影响,负责人:陈妍。

摘　　要：目的由75%食用级无水乙醇建立急性酒精中毒大鼠模型,探究连翘提取物对急性酒精中毒大鼠的作用机制。方法40只大鼠由75%食用级无水乙醇建立急性酒精中毒大鼠模型,成功36只,随机分为模型组、低浓度组(40 mg·L^(-1))、中浓度组(80 mg·L^(-1))、高浓度组(160 mg·L^(-1)),各9只。进行翻正反射实验,生物生化分析仪检测谷丙转氨酶(Alanine aminotransferase,ALT)、谷草转氨酶(Aspartate aminotransferase,AST)水平,免疫投射比浊法检测β-内啡肽(β-Endorphins,β-EP)水平,硫代巴比妥酸法检测丙二醛(malondialdehyde,MDA),2,4-二硝基氟苯法检测谷胱甘肽-S-转移酶(Glutathione S-transferase,GST),酶联免疫法检测P-选择素。Western Blot检测组织特异性抑制因子-1(Tissue specific inhibitor-1,TIMP-1)、缺氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)表达。结果与模型组相比,低浓度组、中浓度组、高浓度组翻正反射消失时间缩短,SOD水平升高,翻正反射恢复时间延长,ALT、AST、MDA、GST、P-选择素、β-EP、TIMP-1、HIF-1α水平降低,有统计学意义(P<0.05)。与低浓度组、高浓度组相比,中浓度组翻正反射消失时间缩短,SOD水平升高,翻正反射恢复时间延长,ALT、AST、MDA、GST、P-选择素、β-EP、TIMP-1、HIF-1α水平降低,有统计学意义(P<0.05)。结论连翘提取物通过调控TIMP-1、HIF-1α表达,改善急性酒精中毒大鼠肝功能,起到抗氧化效果,中浓度连翘提取物作用效果最明显。Objective To explore the mechanism of Forsythia forsythia extract on acute alcoholism rats models established with 75%food grade ethanol.Methods A total of 40 rats were given 75%food grade ethanol to establish alcoholism rat model,and 36 rats were successfully divided into model group,low concentration group(40 mg·L^(-1)),medium concentration group(80 mg·L^(-1))and high concentration group(160 mg·L^(-1)),with 9 rats in each group.Righting reflex experiment was performed.The levels of alanine transaminase(ALT)and aspartate transaminase(AST)were measured by biochemistry analyzer.Immunoprojection nephelometry assay was used to detectβ-Endophilin(β-EP)levels.Malondialdehyde(MDA)was detected by thiobarbituric acid method.Glutathione-S-transferase(GST)was detected by the 2,4-dinitrofluorobenzene method.P-selectin was detected by enzyme-linked immunoassay.Tissue specific inhibitory factor-1(TIMP-1),hypoxia inducible factor-1α(HIF-1α)expression were detected by Western blot.Results Compared with model group,the disappearance time of righting reflex in low concentration group,medium concentration group and high concentration group was shortened.SOD level was increased,righting reflex recovery time was prolonged,and the levels of ALT,AST,MDA,GST,p-selectin,β-EP,TIMP-1 and HIF-1αwere decreased,with statistical significance(P<0.05).Compared with low concentration group and high concentration group,the disappearance time of righting reflex was shortened,SOD level was increased,righting reflex recovery time was prolonged,ALT,AST,MDA,GST,p-selectin,β-EP,TIMP-1,HIF-1αlevels were decreased in medium concentration group,with statistical significance(P<0.05).Conclusions Fructus Forsythia extract can improve the liver function of rats with acute alcoholism by regulating the expression of TIMP-1 and HIF-1α,and has antioxidant effect.The effect of Fructus Forsythia extract at medium concentration is the most obvious.

关 键 词：连翘提取物 急性酒精中毒 组织特异性抑制因子-1 缺氧诱导因子-1α 

分 类 号：R285.5[医药卫生—中药学]