石斛PRMT基因家族的鉴定及其在不同光周期下的表达  

Genome-wide identification of PRMT gene family and expression analysis under different photoperiods in Dendrobium

作　　者：赵桦 张舒婷[1] 刘蒲东 倪珊珊 付帅 叶炜 陈裕坤[1] 林玉玲[1] 徐涵[1] 赖钟雄[1] ZHAO Hua;ZHANG Shuting;LIU Pudong;NI Shanshan;FU Shuai;YE Wei;CHEN Yukun;LIN Yuling;XU Han;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Sanming Institute of Agricultural Sciences,Sanming 365000,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002 [2]三明市农业科学研究院,三明365000

出　　处：《应用与环境生物学报》2022年第2期413-422,共10页Chinese Journal of Applied and Environmental Biology

基　　金：福建省重大科技专项(2015NZ0002-1);福建省高原学科建设经费(102/71201801101);福建农林大学科技创新基金项目(CXZX2017189、CXZX2018076、KF2015108)资助。

摘　　要：蛋白质精氨酸甲基转移酶(protein arginine methyltransferases,PRMTs)是真核生物基因组中重要的表观遗传调控因子之一,在植物信号转导、蛋白亚细胞定位和转录调控中发挥着重要作用.基于铁皮石斛基因组数据库,采用生物信息学方法对石斛PRMT基因家族进行成员鉴定,并对其基因结构、蛋白质保守基序(motif)、蛋白质理化特性和启动子顺式作用元件等进行分析.通过qRT-PCR检测DoPRMT在不同组织部位中的表达模式及对不同光周期条件的响应.在铁皮石斛(Dendrobium officinale)基因组上共筛选鉴定到6个PRMT成员,基因结构分析发现,该家族各成员内含子外显子数量有所差异,其中相差数量最多的达到25个.系统进化树可将石斛PRMT家族划分为3种类型(I、II、III)甲基转移酶,石斛中存在I型PRMT有5个,1个II型PRMT,III型甲基转移酶在其中不存在同源序列.保守结构域分析结果显示,该家族成员含有特定的PRMT和PrmA保守结构域,多数成员仅含有PrmA结构域.保守基序分析发现,该家族包含motif数量较多,各成员包含共有的motif1和motif4.启动子作用元件分析发现,该家族成员包含众多光响应元件及激素应答、厌氧诱导、逆境响应等相关响应元件.qRT-PCR数据表明,该家族各成员在金钗石斛(Dendrobium nobile)叶片中表达较高,茎中的表达较低,少数成员在根中表达较高.此外,DoPRMT在不同光周期下呈现差异性表达模式,各成员在24 h(光照)/0 h(黑暗)下相对表达量较高,在12 h/12 h、8 h/16 h、4 h/20 h等光周期条件下表达相对较低.本研究表明石斛PRMT家族成员表达具有组织特异性,并且可能在石斛响应光周期的过程中发挥重要作用.(图7表4参58)Protein arginine methyltransferases(PRMTs)are one of the important epigenetic regulators in eukaryotic genomes.PRMTs are important in plant signal transduction,protein subcellular localization,and transcriptional regulation.Based on the Dendrobium officinale genome database,bioinformatics methods were used to identify the members of the Dendrobium PRMT gene family.Their gene structure,motif,physicochemical properties of proteins,and promoter cis-acting elements were analyzed.The expression pattern of DoPRMT in different tissues and response to different photoperiod conditions were detected using qRT-PCR.Six PRMTs were screened and identified in the D.officinale genome.Gene structure analysis revealed different numbers of intron exons of each member of the family.Among these,the number of exons differed by as much as 25.Phylogenetic tree analysis found that the Dendrobium PRMT family can divide into three types(I,II,and III).These included five type I PRMTs and one type II PRMT.There were no type III sequences.DoPRMT family members contained specific PRMT and PrmA conserved domains.Most members contained only PrmA domains.Conservative motif analysis revealed that all DoPRMT family members contained motif1 and motif4.Promoter cis-acting element analysis revealed that DoPRMT family members contained light response,hormone response,anaerobic induction,and stress response elements.qRT-PCR results showed that the expression levels of DoPRMT were higher in leaves,lower in stems,with a few members expressed at higher levels in roots.At the same time,DoPRMTs displayed different expression patterns in various photoperiods.The relative expression level of each member was higher under the 24 h(light)/0 h(dark)photoperiod treatment,and relatively low under the 12 h/12 h,8 h/16 h,and 4 h/20 h photoperiod treatments.The collective findings show that the expression of Dendrobium PRMT family members is tissuespecific and may play an important role in the photoperiodic response of Dendrobium.

关 键 词：铁皮石斛 金钗石斛 DoPRMT 表达模式 光周期 

分 类 号：S567.239[农业科学—中草药栽培]