稀土离子对胰蛋白酶的影响  

Effect of Rare Earth Ions on Trypsin

作　　者：柳春霞 时鹏飞 刘建国 Liu Chun-xia;Shi Peng-fei;Liu Jian-guo

机构地区：[1]齐鲁制药(内蒙古)有限公司,内蒙古呼和浩特010080

出　　处：《化工设计通讯》2022年第5期187-189,共3页Chemical Engineering Design Communications

摘　　要：采用酶活测定、紫外光谱探讨了稀土钕离子对胰蛋白酶活性的影响及其机理。实验结果为:不同浓度的稀土钕离子对胰蛋白酶的活性具有抑制作用;紫外吸收光谱检测结果显示稀土钕离子可影响胰蛋白酶的立体结构。实验结果说明稀土钕离子可抑制胰蛋白酶的活性,抑制机理可能是稀土钕离子可改变胰蛋白酶的立体结构,使酶活性丧失。The effect of rare earth neodymium ions on the activity of trypsin and its mechanism were investigated by enzyme activity assay and ultraviolet spectroscopy.The experimental results are:different concentrations of rare earth neodymium ions can inhibit the activity of trypsin;UV absorption spectrum detection results show that rare earth neodymium ions can affect the three-dimensional structure of trypsin.The experimental results show that the rare earth neodymium ions can inhibit the activity of trypsin,and the inhibition mechanism may be that the rare earth neodymium ions can change the three-dimensional structure of trypsin and cause the loss of the enzyme activity.

关 键 词：稀土 钕离子 胰蛋白酶 酶活 紫外光谱 酶蛋白结构 

分 类 号：O629.8[理学—有机化学]