隔药饼灸对动脉粥样硬化易损斑块兔RhoA/ROCK1/MMP-9信号通路的影响  

Effects of Herbal-cake-separated Moxibustion on RhoA/ROCK1/MMP-9 Signaling Pathway in Rabbits with Atherosclerotic Vulnerable Plaque

作　　者：马逸杰 廖意娟 饶泽华 宋家薇 易丽贞 吴雪芬[1] 刘欣 岳增辉[1] MA Yijie;LIAO Yijuan;RAO Zehua;SONG Jiawei;YI Lizhen;WU Xuefen;LIU Xin;YUE Zenghui(College of Acupuncture&Moxibustion and Tui-na,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410208

出　　处：《中国中医药信息杂志》2022年第5期81-86,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81874430)。

摘　　要：目的观察隔药饼灸对动脉粥样硬化(AS)易损斑块兔RhoA/ROCK1/MMP-9信号通路的影响,探讨其稳定AS易损斑块可能的作用机制。方法70只新西兰大耳白兔随机分为空白组(10只)和手术组(60只),采用高脂饲料喂养+腹主动脉球囊损伤+基因转染+药物触发建立AS易损斑块兔模型。将手术组兔随机分为模型组、直接灸组、隔药饼灸组、西药组和抑制剂组,直接灸组和隔药饼灸组取“巨阙”、双侧“天枢”“丰隆”和双侧“心俞”“肝俞”“脾俞”,2组穴位隔日交替施灸,西药组予阿托伐他汀1.96 mg/(kg·d)口服,抑制剂组耳缘静脉注射盐酸法舒地尔10 mg/(kg·d),空白组和模型组只捆绑不干预,连续8周。ELISA检测血清高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量,HE染色观察兔腹主动脉内膜和中膜形态,免疫组化染色检测腹主动脉Ras同源物基因组成员A(RhoA)、Rho相关卷曲形成蛋白激酶1(ROCK1)和基质金属蛋白酶-9(MMP-9)蛋白表达,天狼猩红染色测定胶原纤维含量。结果与空白组比较,模型组兔血清HDL-C含量显著减少(P<0.01),LDL-C含量显著增加(P<0.01);腹主动脉结构紊乱,内膜增厚;腹主动脉组织RhoA、ROCK1和MMP-9蛋白表达显著升高(P<0.01),胶原纤维含量显著减少(P<0.01)。与模型组比较,隔药饼灸组兔血清HDL-C含量显著增加(P<0.05),LDL-C含量显著减少(P<0.01);斑块中细胞形态较模型组更为完整,内膜、中膜界限明显;腹主动脉组织RhoA、ROCK1和MMP-9蛋白表达显著降低(P<0.01),胶原纤维含量显著增加(P<0.01)。结论隔药饼灸可调节AS易损斑块兔HDL-C、LDL-C含量,稳定易损斑块,其作用机制可能与抑制RhoA/ROCK1/MMP-9信号通路、增加胶原纤维含量,进而增加纤维帽厚度有关。Objective To observe the effects of herbal-cake-separated moxibustion on the RhoA/ROCK1/MMP-9 signaling pathway in atherosclerotic vulnerable plaque;To explore its possible mechanism of stabilizing atherosclerotic vulnerable plaque.Methods A total of 70 New Zealand white rabbits were randomly divided into blank group(10 rabbits)and operation group(60 rabbits).A rabbit model of atherosclerotic vulnerable plaques was established by high-fat diet+balloon injury+gene transfection+drug induction.The operation group was randomly divided into model group,direct moxibustion group,herbal-cake-separated moxibustion group,Western medicine group,and inhibitor group.The direct moxibustion group and herbal-cake-separated moxibustion group were applied to“Juque”(RN14),bilateral“Tianshu”(ST25),“Fenglong”(ST40),and bilateral“Xinshu”(BL15),“Ganshu”(BL18),“Pishu”(BL20).Moxibustion applied alternately on acupoints of the two groups every other day.The Western medicine group was given atorvastatin 1.96 mg/(kg•d)orally,and the inhibitor group was given fasudil hydrochloride 10 mg/(kg•d)by the ear vein injection.The blank group and the model group were only bound without intervention.All groups were treated for 8 consecutive weeks.The contents of HDL-C and LDL-C were determined by ELISA;the morphology of the intima and media were observed by HE staining;the expressions of RhoA,ROCK1 and MMP-9 protein in abdominal aorta tissue were detected by immunohistochemistry;the collagen fiber content was determined by picrosirius red staining.Results Compared with the blank group,the content of HDL-C in serum of the rabbits in model group was significantly reduced(P<0.01),the content of LDL-C significantly increased(P<0.01).The abdominal aorta structure was disordered,the intima was thickened.The expressions of RhoA,ROCK1 and MMP-9 protein in abdominal aortic tissue significantly increased(P<0.01),and the content of collagen fibers significantly reduced(P<0.01).Compared with the model group,the content of HDL-C in seru

关 键 词：隔药饼灸 动脉粥样硬化 易损斑块 RhoA/ROCK1/MMP-9信号通路 兔 

分 类 号：R245.8[医药卫生—针灸推拿学]