巴山木竹开花前后植物内源激素变化研究  

Changes of endogenous hormones in Bashania fargesii in the process of blooming

作　　者：杜喜春[1] 何祥博 赵银萍[1] 张九东[1] 郭成圆 DU Xi-chun;HE Xiang-bo;ZHAO Yin-ping;ZHANG Jiu-dong;GUO Cheng-yuan(School of Biological and Environmental Engineering,Xi’an University,Xi’an 710065,Shaanxi,China;The Administration of Shaanxi Foping National Nature Reserve,Foping 723400,Shaanxi,China)

机构地区：[1]西安文理学院生物与环境工程学院,陕西西安710065 [2]陕西省佛坪国家级自然保护区管理局,陕西佛坪723400

出　　处：《云南大学学报（自然科学版）》2022年第3期612-618,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：陕西省自然科学基础研究计划(2018JM3029).

摘　　要：为了解巴山木竹开花过程中植物内源激素的变化状况,研究依次选取3个不同生长发育阶段(营养生长阶段、开花前期阶段、开花阶段)的巴山木竹叶片,运用酶联免疫分析法测定其内源激素水平.结果表明,随着生长发育时期的推进,生长素质量比逐步升高,在营养生长阶段质量比最低,开花阶段最高,竹叶中生长素的质量比可以作为预测竹子开花的预警指标;赤霉素质量比出现先降低,然后升高的趋势,因此推测赤霉素途径可能不是巴山木竹的主要开花途径,但该激素在开花前降低的现象仍可作为预测竹子开花的预警指标;细胞分裂素的质量比变化和赤霉素基本一致,其开花前降低的现象也可作为预测竹子开花的预警指标,同时根据其作用机理,推测年龄途径是巴山木竹开花的可能途径,这一点还需要后续的实验进一步证实;脱落酸质量比在开花前期变化不大,开花时才显著升高.从营养阶段到开花前期,叶片中叶绿素相对含量和氮相对含量会降低;进入开花期后,叶绿素相对含量和氮相对含量又小幅升高.研究可为进一步研究巴山木竹的开花机制提供参考,对于保护秦岭大熊猫也具有一定的意义.In order to understand the changes of plant endogenous hormones in the process of flowering,the leaves of Bashania fargesii were selected from different growth stages(vegetative growth stage,pre-flowering stage,flowering stage)in turn.The level of endogenous hormones in the leaves of Bashania fargesii was determined by enzyme-linked immunosorbent assay(ELISA).The results showed that the concentration of IAA was gradually increased in its growing and developing process,the concentration was lowest in vegetative growth stage and highest in flowering stage.The concentration of IAA can be used as the early warning indicator for predicting the flowering of bamboo.The concentration of GA was decreased in the pre-flowering stage,and increased again in the flowering stage,indicates that GA pathway may not be the main flowering pathway of Bashania fargesii.However,the decrease of this hormone before flowering can still be used as an early warning indicator for predicting bamboo flowering.The change of concentration of the CTK was similar with GA in the whole process of blooming,it can be also used as early warning index to predict bamboo flowering.According to its mechanism,it is suggested that age pathway may be the main way of flowering of bamboos.The concentration of ABA did not change much in the pre-flowering stage,but increased significantly in the flowering stage.The contents of chlorophyll and nitrogen decreased from the vegetative stage to the bud initiation and development stage,and increased slightly in the flowering stage.This study can provide reference for further study on flowering mechanism of the Bambusa fargesii,also,it has significance for the protection of the giant pandas in the Qinling Mountains.

关 键 词：巴山木竹 植物内源激素 生长素 细胞分裂素 赤霉素 脱落酸 

分 类 号：S795[农业科学—林木遗传育种]