双孢线虫草真菌群落结构及多样性分析  

Diversity and structure analysis of fungal community on Ophiocordyceps bispora

作　　者：汤德相 王笑研 王元兵 孙涛[1,2] 胡卫红 虞泓[1,2] TANG De-xiang;WANG Xiao-yan;WANG Yuan-bing;SUN Tao;HU Wei-hong;YU Hong(Yunnan Herbal Laboratory,School of Ecology and Environmental Science,Yunnan University,Kunming,650091,Yunnan,China;The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia,Yunnan University,Kunming 650091,Yunnan,China;School of Life Sciences,Yunnan University,Kunming,650091,Yunnan,China)

机构地区：[1]云南大学生态学与环境学院云南云百草实验室,云南昆明650091 [2]云南大学中国(云南)−东南亚虫草生物资源可持续利用国际联合研究中心,云南昆明650091 [3]云南大学生命科学学院,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2022年第3期619-626,共8页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省科技厅−云南大学联合基金(2018FY001(-006));国家自然科学基金(31760011,31870017).

摘　　要：双孢线虫草Ophiocordyceps bispora是线虫草属Ophiocordyceps古老的物种.非生物因子与微生物对其生长发育和适应环境具有重要作用.基于Illumina MiSeq高通量测序技术对5组双孢线虫草样本进行ITS2(Internal Transcribed Spacer 2)区域测序,以比较不同海拔梯度和光照强度下真菌多样性、丰富度和群落结构.测序共得到445个OTUs,经鉴定共有3门、13纲、41目、69科、66属和257种真菌物种.其中子囊菌门为优势门,线虫草属为优势属.α多样性指数与海拔梯度相关性分析发现,真菌群落多样性和丰富度与海拔梯度不存在显著相关性,光照强度、植被类型和人为活动可能是影响真菌群落丰富度和多样性的主要环境因子.真菌群落和海拔梯度相关性分析表明,其中14个属与海拔梯度呈正相关.同时,Anosim分析发现双孢线虫草真菌群落组间存在显著差异.以上研究结果表明,双孢线虫草蕴藏着丰富的真菌资源,海拔对其群落结构具有一定影响,但对其丰富度和多样性影响较小,推测光照强度、人为活动和植被类型可能是影响其真菌群落结构、丰富度以及多样性的主要环境因素.该研究为双孢线虫草微生物群落中真菌资源的挖掘与利用提供参考,同时为虫草真菌群落与环境相互作用提供研究思路.Ophiocordyceps bispora was a species of the genus Ophiocordyceps.Abiotic factors and microorganisms play important roles in the growth,development and adaptation of Ophiocordyceps bispora.In this study,five group samples of Ophiocordyceps bispora were sequenced by region(ITS2)Illumina MiSeq high-throughput sequencing technology,in order to compare fungal diversity,richness and community structure under different altitude gradients and light intensities.A total of 445 OTUs were gained,and 3 phyla,13 classes,41 orders,69 families,66 genera and 257 fungal species were identified.Ascomycetes was the dominant phylum and Ophiocordyceps was the dominant genus.The correlation analysis between Alpha diversity index and altitude gradient showed that there were no significant correlation between fungal community diversity and richness and altitude gradient.Light intensity,vegetation type and human activities might be the main environmental factors affecting fungal community richness and diversity.The correlation analysis between fungal community and altitude gradient showed that 14 genera were positively correlated with altitude gradient.At the same time,Anosim analysis found that there were significant differences among the groups of Ophiocordyceps bispora fungal communities.The above results showed that Ophiocordyceps bispora contains rich in fungal resources.Altitude had a certain impact on Ophiocordyceps bispora community structure,but has little impact on the richness and diversity of Ophiocordyceps bispora.It is speculated that light intensities,human activities and vegetation types might be the main environmental factors affecting Ophiocordyceps bispora fungal community structure,richness and diversity.This study provided a reference for the excavation and utilization of fungal resources in Ophiocordyceps bispora microbial communities,and provided a research idea for the interaction between Cordyceps fungal communities and environment.

关 键 词：双孢线虫草 群落结构 多样性 海拔梯度 光照强度 

分 类 号：Q93[生物学—微生物学]