外源一氧化氮(NO)对干旱胁迫滇润楠幼苗生长及生理特性的影响  

Effects of exogenous nitric oxide(NO)on growth and physiological characteristics of Machilus yunnanensis seedlings under drought stress

作　　者：宁朋 李燕 张敏[1] 王菲 黄晓霞[1] NING Peng;LI Yan;ZHANG Min;WANG Fei;HUANG Xiao-xia(College of landscape Architecture and Horticulture,Southwest Forestry University,Southwest Landscape Architecture Engineering Research Center of State Forestry and Grassland Administration,Kunming 650224,Yunnan,China)

机构地区：[1]西南林业大学园林园艺学院,国家林业与草原局西南风景园林工程技术研究中心,云南昆明650224

出　　处：《云南大学学报（自然科学版）》2022年第3期636-646,共11页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(31760197).

摘　　要：以硝普钠(SNP)为外源一氧化氮(NO)供体,探究外源NO对干旱环境下滇润楠(Machilus yunnanensis)幼苗生长及生理特性的影响,为当前水分匮乏背景下城市园林树种抗旱能力的提高提供一定的理论基础.结果显示:①干旱胁迫下滇润楠幼苗的叶片相对含水量(LRWC)、叶绿素(Chl)质量比、生物量和光合速率(Pn)显著降低,膜脂过氧化产物丙二醛(MDA)含量和抗氧化酶活性显著增加;②SNP处理后,滇润楠幼苗的Chl、脯氨酸(Pro)、可溶性蛋白(SP)和可溶性糖(SS)含量增加,LRWC、Pn、生物量和抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均升高,MDA含量显著降低;③在所有SNP处理组中,叶面喷施处理的作用效果要好于根际浇灌处理;在叶面喷施的处理组中,以0.1 mmol/L的SNP的处理作用效果最好,在SNP浓度为0.2 mmol/L时作用效果减弱.研究表明:干旱胁迫下滇润楠幼苗的生长和光合能力受到抑制,膜脂受到氧化损伤,同时干旱胁迫也提高了滇润楠幼苗的根冠比(R/S)、渗透调节物质含量和抗氧化酶活性;外源NO处理进一步提高了滇润楠幼苗的渗透调节物质含量和抗氧化酶活性,有效提高滇润楠的抗旱能力,维持其在干旱环境下的生物量积累和正常生长;外源NO的作用效果受施用方式和施用浓度的影响,在适宜浓度下可以显著提高滇润楠幼苗的抗旱性.Using sodium nitroprusside(SNP)as an exogenous nitric oxide(NO)donor,the effect of exogenous NO on the growth and physiological characteristics of Machilus yunnanensis seedlings under drought stress were investigated,it provides a theoretical basis for improving the drought resistance of landscape plants in urban green space under the current water shortage.The results showed that:①Under drought stress,the relative water content of leaves(LRWC),the chlorophyll content(Chl)and the biomass and photosynthetic rate(Pn)in leaves of Machilus yunnanensis seedlings were significantly decreased,and the content of malondialdehyde(MDA)and the activities of antioxidant enzymes were significantly increased;②after treatment with SNP,the contents of Chl,Pro,SP,SS of Machilus yunnanensis seedlings were increased,the LRWC,Pn and the biomass of Machilus yunnanensis seedlings were improved,and the activities of ascorbic acid peroxidase(APX),peroxidase(POD),catalase(CAT)of Machilus yunnanensis seedlings were enhanced,while the content of MDA was decreased significantly;③among all SNP treatments,the effect of foliar spraying was better than that of rhizosphere irrigation,among the foliar spray treatment group,0.1 mmol/L SNP had the best effect,and the effect was weakened when the concentration of SNP was 0.2 mmol/L.The study showed that the growth and photosynthesis of Machilus yunnanensis seedlings were inhibited,the membrane lipid of Machilus yunnanensis seedlings was damaged under drought stress,drought stress also increased the root-shoot ratio(R/S),the osmotic adjustment substance content and the antioxidant enzymes activities of Machilus yunnanensis seedlings.The exogenous NO treatment further increased the osmotic adjustment substance content and the antioxidant enzymes activities of Machilus yunnanensis seedlings,and effectively enhanced drought resistance of Machilus yunnanensis,and maintained its biomass accumulation and normal growth under drought condition.The effect of exogenous NO is affected by the application me

关 键 词：滇润楠 干旱胁迫 外源一氧化氮 

分 类 号：Q945.78[生物学—植物学]