清开灵注射液延长血小板体外保存期对其功能影响的研究  

Effect of Qingkailing injection on platelets function preserved in vitro

作　　者：李洁 张雪睿[1] 冯文斌[1] 黄雁[2] 王珺 王云龙 谭小霞 兰炯采[3] LI Jie;ZHANG Xuerui;FENG Wenbin;HUANG Yan;WANG Jun;WANG Yunlong;TAN Xiaoxia;LAN Jiongcai(Department of Transfusion Medicine,Beihai People’s Hospital,Beihai 536000,China;Beihai Blood Center;Nanfang Hospital,Southern Medical University)

机构地区：[1]北海市人民医院输血科,广西北海536000 [2]北海市中心血站 [3]南方医科大学南方医院

出　　处：《中国输血杂志》2022年第4期387-391,共5页Chinese Journal of Blood Transfusion

基　　金：广西壮族自治区北海市科技局项目(2019C05)。

摘　　要：目的探讨清开灵注射液对体外保存血小板功能的影响。方法取15(人)份机采血小板(≥250 mL/份),添加终浓度为1%清开灵注射液约1.25 mL/份,作为实验组22℃振荡保存,于保存d1、d3、d5、d8、d10和d14抽取标本,检测血小板的活化指标血栓弹力图(TEG)﹑CD62p表达率、凋亡指标线粒体膜电位(JC-1);同时设做对照组:取与实验组等量(份)机采血小板、同保存条件,于保存d1、d3和d5抽取标本做相同项目的检测。比较2组各检测指标的差异。结果1)实验组保存d1~d14 TEG的K、α值在组内无明显变化(P>0.05);R值(min)由6.12±1.58增至11.02±2.26、CI值由0.27±1.24增至-10.47±3.51(P<0.05);MA值(mm)保存至d5时组内(间)尚无明显改变(P>0.05),至d8始降至53.18±2.71、至d14为22.88±3.45(P<0.05)。对照组保存d1~d5 TEG各指标,K值、A角无明显改变(P>0.05),R时明显延长,MA距减小,CI(绝对)值明显增加(P<0.05)。2)CD62p表达率(%):保存d5时实验组与对照组为70.50±9.12 vs 83.16±5.33(P<0.05),d14时为82.77±7.17(P<0.05)。3)JC-1(%):2组内血小板在常规保存期内无明显改变(P>0.05),只是d5时实验组与对照组为86.75±3.88 vs 70.36±19.8(P<0.05);实验组d8、d10、14d时分别为81.04±9.50 vs 71.38±8.77 vs 82.77±7.17(P<0.05)。结论添加1%清开灵注射液的机采血小板体外保存至14 d时的血小板活化、聚集及抗凋亡功能,与常规保存血小板相近。Objective To investigate the effect of Qingkailing injection on platelet function preserved in vitro.Methods A total of 15 bags of plateletpheresis(≥250 mL/bag),adding Qingkailing injection(1.25 mL/bag)at the final concentration of 1%,were stored at 22℃with gentle agitation as the experimental group,and samples were collected on day 1,3,5,8,10 and 14 to detect the thromboelastogram(TEG),CD62 p expression rate and mitochondrial membrane potential(JC-1).The control group was set up synchronously,with the same volume and storage conditions as the experimental groups,and samples were taken on day 1,3 and 5 after preservation to undertake the same test items as the experimental groups.The differences of detection indexes between the two groups were compared.Results 1)In the experimental group,there was no significant change in K andαduring day 1 to 14(P>0.05).The R value(min)increased from 6.12±1.58 to 11.02±2.26,and the CI value changed from 0.27±1.24 to-10.47±3.51(P<0.05).The MA value(mm)had no significant change within 5 days(P>0.05),but decreased to 53.18±2.71 on day 8 and 22.88±3.45 on day 14(P<0.05).In the control group during day 1 to 5,K(min)andαshowed no significant change,while R(min)was significantly prolonged,MA(mm)decreased and CI increased significantly(P<0.05).2)The expression rate of CD62 p(%)was 70.50±9.12 in the experimental group on day 5(vs 83.16±5.33 in the control groups,P<0.05)and 82.77±7.17 on day 14(P<0.05).3)There was no significant change in JC-1(%)during regular preservation period between the experimental group and control group(P>0.05),but JC-1(%)was 86.75±3.88 vs 70.36±19.8 on day 5(P<0.05).In the experimental group,JC-1(%)was 81.04±9.50 vs 71.38±8.77 vs 82.77±7.17 on day 8,10 and 14,respectively.Conclusion The activation and aggregation as well as anti-apoptosis function of plateletpheresis,adding Qingkailing injection at the final concentration of 1%,are similar to those of routine preservation.

关 键 词：清开灵注射液 血小板体外保存 血小板功能 血小板活性 

分 类 号：R457.12[医药卫生—治疗学] R331.143[医药卫生—临床医学]