5-氮杂胞苷调节新型冠状病毒肺炎病毒RNA甲基化研究  

5-Azacytidine Regulates RNA Methylation in COVID-19 Virus

作　　者：石明明 唐冲 辛颖[1] Shi Mingming;Tang Chong;Xin Ying(College of Basic Medical Sciences,Jilin University,Changchun 130021;BGI Shenzhen,Shenzhen 518083)

机构地区：[1]吉林大学基础医学院,长春130021 [2]深圳华大基因,深圳518083

出　　处：《国际老年医学杂志》2022年第3期263-267,共5页International Journal of Geriatrics

基　　金：吉林省科技厅中青年科技创新创业卓业团队项目(20210509003RQ)。

摘　　要：目的使用表观遗传疗法,通过5-氮杂胞苷调节严重急性呼吸系统综合征冠状病毒2(SARS-CoV-2)RNA的甲基化来抑制病毒的复制,从而为新型冠状病毒肺炎(COVID-19)提供新的潜在治疗方案。方法包装假病毒VSV-SARS-CoV-2-Sdel18感染BHK21-hACE2细胞,检测5-氮杂胞苷对病毒的抑制效果,并与氯喹进行比较。对病毒基因组RNA进行甲基化测序,统计其5-甲基胞嘧啶水平。结果经5-氮杂胞苷处理的细胞显示出显著降低的荧光强度。5-氮杂胞苷处理的样品中5-甲基胞嘧啶水平降低了15%。结论5-氮杂胞苷能够减少SARS-CoV-2病毒RNA的甲基化,从而有效地抑制病毒复制。Objective To investigate the inhibitory effect of 5-azacytidine on coronavirus replication by regulating the methylation of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) RNA.Methods BHK21-hACE2 cells were infected with pseudovirus VSV-SARS-CoV-2-Sdel18.The inhibitory effect of 5-azacytidine on the virus was tested and compared with that of chloroquine.The viral genomic RNA was analyzed by methylation sequencing and the level of 5-methylcytosine was determined.Results 5-Azacytidine-treated cells showed significantly reduced fluorescence intensity.The level of 5-methylcytosine was significantly reduced by 15% in 5-azacytidine-treated cells.Conclusion 5-Azacytidine may effectively inhibit virus replication by reducing the methylation of SARS-CoV-2 RNA.

关 键 词：新型冠状病毒肺炎 5-氮杂胞苷 RNA甲基化 

分 类 号：R373[医药卫生—病原生物学]