绵羊源矛形双腔吸虫形态学观察和PCR鉴定  

Morphological and PCR-based Identification of Dicrocoelium Dendriticum Isolated from Sheep

作　　者：邓金华 王云峰 齐萌[1] 邓金珠玛 余复昌 DENG Jinhua;WANG Yunfeng;QI Meng;DENG Jinzhuma;YU Fuchang(College of Animal Science,Tarim University,Alar 843300,China;Bayinguoleng Mongolia Autonomous Prefecture Center for Control and Diagnosis of Animal Diseases,Kurle 841000,China)

机构地区：[1]塔里木大学动物科学学院,阿拉尔843300 [2]巴音郭楞蒙古自治州动物疾病控制与诊断中心,库尔勒841000

出　　处：《中国草食动物科学》2022年第3期73-76,共4页China Herbivore Science

基　　金：兵团中青年科技创新领军人才计划项目(2018CB034);新疆生产建设兵团重点领域科技攻关计划项目(2020AB025)。

摘　　要：为鉴定新疆和静县绵羊感染的双腔吸虫种类,分析其遗传进化特征,采用形态学观察和PCR法相结合的方法,对和静县绵羊肝胆管内分离出来的双腔吸虫进行了种类鉴定和序列分析。结果显示,经形态学观察,初步鉴定绵羊感染的吸虫为矛形双腔吸虫;基于吸虫的ITS序列,采用PCR法扩增该虫的DNA样本,测序所得序列与山西省周至县山羊源矛形双腔吸虫序列的同源性为100%,鉴定为矛形双腔吸虫。To identify the species of Dicrocoelium in sheep in Hejing county,morphological observation and PCR identification were performed on the Dicrocoelium isolated from the hepatobiliary duct of sheep in Hejing county.The results showed that the Dicrocoelium isolate was initially identified as a Dicrocoelium dendriticum according to the morphological characteristics.The DNA sample was amplified by PCR based on the ITS sequence,and the nucleotide sequence obtained showed 100%homology with KC774500(sheep-derived D.dendriticum).Thus the Dicrocoelium isolate was identified as D.dendriticum.

关 键 词：矛形双腔吸虫 形态学观察 种类 PCR 

分 类 号：S858.26[农业科学—临床兽医学]