锦绣杜鹃类黄酮-3′,5′-羟基化酶基因的生物信息学分析  

Bioinformatics analysis of flavonoid-3′,5′-hydroxylase gene from Rhododendron pulchrum

作　　者：孙世宇 冯猜 孙威[1,2] 徐小蓉 SUN Shiyu;FENG Cai;SUN Wei;XU Xiaorong(Key Laboratory of Plant Physiology and Development Regulation/School of Life Science, Guizhou Normal University,Guiyang,Guizhou 550025,China;Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountain Area of Southwest of China,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州师范大学生命科学学院/植物生理与发育调控重点实验室,贵州贵阳550025 [2]西南喀斯特山地生物多样性保护重点实验室,贵州贵阳550025

出　　处：《贵州师范大学学报（自然科学版）》2022年第3期33-38,55,共7页Journal of Guizhou Normal University：Natural Sciences

基　　金：国家自然科学基金(31760076);贵州省教育厅特色领域项目(黔教合KY字[2021]059);贵州省科技厅科技支撑计划项目(黔科合支撑[2020]4Y028号);贵州省科技厅基础研究项目(黔科合基础[2019]1019);贵州省科技计划(黔科合平台人才[2017]5726号)。

摘　　要：类黄酮-3′,5′-羟基化酶(Flavonoid 3′,5′-hydroxylase,F3′5′H)是类黄酮代谢途径的一个关键酶,能使花色素的合成方向趋向于蓝色的飞燕草素。利用生物信息学软件对锦绣杜鹃类黄酮-3′,5′-羟基化酶基因(RpF3′5′H)进行分析。结果显示,RpF3′5′H基因ORF长1551 bp,共编码516个氨基酸;分子量为57.31 kD,等电点为9.03,无信号肽,定位于细胞质中的可能性最大,二级结构与三级结构中含有较多的α螺旋,与高丛越橘有较高亲缘性。分析结果将对RpF3′5′H的功能解析奠定理论基础,同时也为探究锦绣杜鹃花色苷的生物合成有一定指导作用。Flavonoid 3′,5′-hydroxylase(F3′5′H)is a key enzyme in flavonoid metabolism pathway,which can make the biosynthesis of anthocyanins tend to blue delphinidin.F3′5′H gene of Rhododendron pulchrum was analyzed by bioinformatics software.The results showed that ORF of RpF3′5′H gene was 1551 bp,encoding 516 amino acids,with a molecular weight of 57.31 KD and an isoelectric point of 9.03.Signal peptide was not detected in RpF3′5′H and it was likely located in the cytoplasm.There were majorα-helix in the secondary and tertiary structures of RpF3′5′H,meanwhile,RpF3′5′H had high affinity with blueberry.The results will lay a theoretical foundation for the functional analysis of RpF3′5′H,and also have a certain guiding role in the study of anthocyanin biosynthesis of Rhododendron pulchrum.

关 键 词：锦绣杜鹃 类黄酮-3′5′-羟基化酶 生物信息学分析 

分 类 号：S685[农业科学—观赏园艺]