多酶共固定化技术在糖类催化中的研究进展  

Advances of multienzymes co-immobilization technology for sugar catalysis

作　　者：唐婷 周文凤 王志[1] 朱晨杰 许敬亮 庄伟[2] 应汉杰[2] 欧阳平凯 TANG Ting;ZHOU Wenfeng;WANG Zhi;ZHU Chenjie;XU Jingliang;ZHUANG Wei;YING Hanjie;OUYANG Pingkai(Biology+Joint Research Centre,Department of Chemical Technology,Zhengzhou University,Zhengzhou 450002,Henan,China;National Engineering Research Center for Biotechnology,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,Jiangsu,China)

机构地区：[1]郑州大学化工学院,生物+联合研究中心,河南郑州450002 [2]南京工业大学生物与制药工程学院,国家生化工程技术研究中心,江苏南京211816

出　　处：《化工进展》2022年第5期2636-2648,共13页Chemical Industry and Engineering Progress

基　　金：国家重点研发计划(2019YFD1101204);国家自然科学基金(21878142);江苏省杰出青年自然科学基金(BK20190035);江苏省重点研发计划(BE2020712);江苏省六大人才高峰项目(SWYY-016)。

摘　　要：糖类的催化反应对人体生命活动和工业生产具有重要意义。近年来,多酶共固定化技术迅速发展,为糖类的催化反应提供了一个绿色高效的研究工具。本文结合糖类催化中的多酶级联反应,系统阐述了多酶共固定化的方法,包括传统方法和新型方法,并指出了其各自的原理和优缺点。在此基础上,详细介绍了多酶共固定化技术在糖类催化中的应用,如淀粉的水解、纤维素的利用以及功能性糖的合成等,结果表明多酶共固定化技术不仅具有高效催化、稳定性强、易于分离等特性,而且能够充分发挥多酶之间协同催化的优势,极大地促进了糖类催化反应的进行。最后分析了多酶共固定化技术目前存在的问题与挑战,提出了一些解决问题的研究思路和方法,并展望了其发展前景。Apparently,the catalytic reaction of sugar is of great significance to human life activities and industrial production.In recent years,the rapid development of the multienzymes co-immobilization technology provides a green and efficient research tool for the catalytic reaction of sugar.Under this background,combined with the multienzymes cascade reaction in sugar catalysis,this paper systematically expounds the traditional and new methods of multienzymes co-immobilization,and illustrates its respective principles and advantages as well as disadvantages.On this basis,this paper further introduces the wide applications of multienzymes co-immobilization technology in sugar catalysis in detail,such as starch hydrolysis,cellulose utilization and functional sugar synthesis.In accordance with the related research results,it is indicated that the multienzymes co-immobilization technology not only has a series of remarkable characteristics including high efficiency catalysis,strong stability and easy separation,but also can give full play to the advantages of synergistic catalysis among multienzymes,thus greatly promoting the catalytic reaction of sugars.In the last,this paper analyzes some of the problems and challenges facing multienzymes co-immobilization technology,puts forward some research thoughts and methods for solving these problems,and looks forward to its development prospects.

关 键 词：多酶共固定化 糖类催化 级联反应 协同效应 

分 类 号：Q814.2[生物学—生物工程]