幽门螺杆菌感染对舌苔菌群的影响  

The human tongue coating microbial community with and without Helicobacter pylori infection

作　　者：包玲珊 余颖聪[1] BAO Ling-shan;YU Ying-cong(Department of Gastroenterology,Wenzhou No.3Clinical Institute Affiliated to Wenzhou Medical University,Wenzhou People′s Hospital,Wenzhou,Zhejiang 325000,China;不详)

机构地区：[1]温州医科大学,温州医科大学第三临床学院温州市人民医院,浙江温州325000

出　　处：《中国微生态学杂志》2022年第3期273-277,共5页Chinese Journal of Microecology

基　　金：温州市中医药建设项目(2018008)。

摘　　要：目的探讨慢性萎缩性胃炎患者和慢性浅表性胃炎患者胃内幽门螺杆菌(Helicobacter pylori,H.pylori)感染与舌苔菌群的关系。方法根据61名患者内镜、病理和H.pylori检测结果,将其分成慢性萎缩性胃炎H.pylori阳性组(12名)、慢性萎缩性胃炎H.pylori阴性组(16名)、慢性浅表性胃炎H.pylori阳性组(8名)和慢性浅表性胃炎H.pylori阴性组(25名)。采集舌苔样本进行16SrRNA基因测序,分析各组患者舌苔菌群结构。结果16SrRNA基因测序结果显示,此次测序样本数据量足够,样本所含物种的丰富程度和均匀程度合理。4组舌苔菌群样本中Observed species指数差异有统计学意义(H=10.0233,P<0.05)。门水平上,舌苔菌群由拟杆菌门、厚壁菌门、变形菌门3大优势菌门组成。属水平上,4组间共有11种菌属的丰度差异有统计学意义(均P<0.05)。在慢性萎缩性胃炎患者中,H.pylori阳性的患者拟普雷沃菌属相对丰度显著低于H.pylori阴性患者,而罗氏菌属的相对丰度则显著升高。此外,61例舌苔菌群样本中,仅1例检测到H.pylori。结论H.pylori感染与舌苔菌群结构存在相关性,然而胃内与口腔中H.pylori的具体关联还需要进一步研究。Objective To explore the relationship between Helicobacter pylori(H.pylori)infection and tongue coating microbial community in patients with chronic atrophic gastritis(CAG)and chronic superficial gastritis(CSG).Methods The 61subjects were divided into CAG with H.pylori+group(n=12),CAG with H.pylori-group(n=16),CSG with H.pylori+group(n=8)or CSG with H.pylori-group(n=25).16SrRNA gene sequencing technology was used to analyze the structure of tongue coating flora in each group.Results The richness and evenness of the species contained in the samples were reasonable,and there were differences in observed species index between the four groups(H=10.0233,P<0.05).At the phylum level,the five dominant bacteria were Bacteroidetes,Firmicutes,Proteobacteria,Fusobacteria and Actinobacteria.At the genus level,a total of 11species of bacteria were statistically different among the four groups(all P<0.05).In patients with CAG,the relative abundance of Alloprevotella was significantly lower in the patients with H.pylori+than in those with H.pylori-,while the relative abundance of Rothia was significantly higher.In addition,only one of the 61tongue coating samples was detected as H.pylori positive.Conclusion There is an association between H.pylori infection and bacterial structure of tongue coating microbiota.The relationship between H.pylori in the stomach and the presence of H.pylori in the tongue coating needs be further studied.

关 键 词：慢性萎缩性胃炎 慢性浅表性胃炎 幽门螺杆菌 舌苔 菌群 

分 类 号：R37[医药卫生—病原生物学]