我国西北地区蚊虫标本中广平病毒的检测  

Detection of Quang Binh virus from mosquitoes in northwestern China

作　　者：李樊 殷启凯 胡伟军[3] 周莉薇[4] 付士红[1,2] 张少白 韩坤[4] 聂凯[1,2] 何英[1,2] 许晓光 许松涛[1,2] 梁国栋 王环宇 Li Fan;Yin Qikai;Hu Weijun;Zhou Liwei;Fu Shihong;Zhang Shaobai;Han Kun;Nie Kai;He Ying;Xu Xiaoguang;Xu Songtao;Liang Guodong;Wang Huanyu(Department of Arboviruses,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;State Key Laboratory for Infectious Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Ningxia Hui Autonomous Region Center for Disease Control and Prevention,Yinchuan 710054,Ningxia,China;Shaanxi Provincial Center for Disease Control and Prevention,Xi'an 750004,Shaanxi,China;Research Center of High Altitude Medicine,People's Hospital of Naqu Affiliated to Dalian Medical University,Naqu 852000,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所虫媒室,北京102206 [2]中国疾病预防控制中心传染病预防控制国家重点实验室,北京102206 [3]陕西省疾病预防控制中心,陕西西安710054 [4]宁夏回族自治区疾病预防控制中心,宁夏银川750004 [5]西藏自治区那曲市人民医院高原医学研究中心,西藏那曲852000

出　　处：《疾病监测》2022年第3期373-376,共4页Disease Surveillance

基　　金：国家自然科学基金(No.31900156);宁夏回族自治区重点研发计划(No.2019BCG01003);中央引导地方项目(No.XZ202001YD0005C)。

摘　　要：目的对我国西北地区蚊虫携带病毒开展调查,并快速发现重要的蚊媒病毒。方法通过形态学方法对采集蚊虫进行分类鉴定,深度测序和宏基因组分析方法分析蚊虫携带的病毒,结合Sanger测序法补全病毒基因组全序,应用系统进化分析方法分析病毒分子遗传特征。结果2019年7月采集自陕西省的中华按蚊标本和宁夏回族自治区的三带喙库蚊标本均检测到黄病毒属广平病毒。进化分析结果显示,这两株广平病毒序列与在中国辽宁省发现的广平病毒JM17156株具有较近亲缘关系,核酸序列同源性为99.8%。结论本研究首次在我国西北地区中华按蚊、三带喙库蚊标本中检测到广平病毒。Objective To investigate the diversity of mosquito-borne virus in northwestern China,and identify important mosquito-borne viruses.Methods The mosquitoes were identified according to morphological methods,the mosquito-borne virus was identified by deep sequencing and metagenomic analysis.Molecular genetic characteristics of identified virus were analyzed by phylogenetic method.Results Quang Binh virus was detected from Anopheles sinensis collected in Shaanxi province and Culex tritaeniorhynchus collected in Ningxia Hui autonomous region in July 2019.Phylogenetic analysis showed that the two strains shared the same evolutionary branch with the JM17156 strain of Quang Binh virus identified in Liaoning province,China,with nucleotide sequence homologous of 99.8%.Conclusion Quang Binh Virus were first successfully identified from Anopheles sinensis and Culex tritaeniorhynchus in Northwestern China.

关 键 词：广平病毒 黄病毒 虫媒病毒 深度测序 宏基因组 

分 类 号：R211[医药卫生—中医学] R373.3