Driving force of biomolecular liquid–liquid phase separation probed by nuclear magnetic resonance spectroscopy  

作　　者：Hanyu Zhang Weiwei Fan Gilbert Nshogoza Yaqian Liu Jia Gao Jihui Wu Yunyu Shi Xiaoming Tu Jiahai Zhang Ke Ruan 

机构地区：[1]Ministry of Education Key Laboratory for Membraneless Organelles&Cellular Dynamics,Hefei National Laboratory for Physical Sciences at the Microscale,School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,Anhui,China [2]Department of Applied Chemistry,College of Science and Technology,University of Rwanda,Kigali,Rwanda

出　　处：《Biophysics Reports》2022年第2期90-99,共10页生物物理学报（英文版）

基　　金：the Ministry of Science and Technology of China (2019YFA0508400 and 2016YFA0500700);the National Natural Science Foundation of China (21874123 and 32071220);the Collaborative Innovation Program of Hefei Science Center, CAS (2020HSC-CIP009), for financial support

摘　　要：The assembly of biomolecular condensates is driven by liquid–liquid phase separation. To understand the structure and functions of these condensates, it is essential to characterize the underlying driving forces, e.g., protein–protein and protein–RNA interactions. As both structured and low-complexity domains are involved in the phase separation process, NMR is probably the only technique that can be used to depict the binding topology and interaction modes for the structured and nonstructured domains simultaneously. Atomic-resolution analysis for the intramolecular and intermolecular interactions between any pair of components sheds light on the mechanism for phase separation and biomolecular condensate assembly and disassembly. Herein, we describe the procedures used for the most extensively employed NMR techniques to characterize key interactions for biomolecular phase separation.

关 键 词：Membraneless organelles Liquid–liquid phase separation NMR spectroscopy Chemical shift perturbation Paramagnetic relaxation enhancement 

分 类 号：Q503[生物学—生物化学]