微小RNA-155与X综合征患者冠状动脉微循环阻力的关系  

Relationship between microRNA-155 and coronary microcirculation resistance in patients with syndrome X

作　　者：赵斌 刘震 雷晓明 ZHAOBin;LIU Zhen;LEI Xiao-ming(Critical Care Unit of Department of Cardiology,The Second Affiliated Hospital of South China University of Technology,Guangzhou First People′s Hospital,Guangzhou 510000,China)

机构地区：[1]华南理工大学附属第二医院广州市第一人民医院心内科CCU,广东广州510000

出　　处：《解剖学研究》2022年第2期146-150,共5页Anatomy Research

基　　金：广州市卫生健康科技一般引导项目(20221A010011);广州市科技计划项目(201804010463)。

摘　　要：目的探讨循环微小RNA-155(miR-155)和氧化应激相关因子与X综合征患者冠状动脉微循环的关系。方法将广州市第一人民医院2018年1月至2020年12月期间住院的X综合征患者进行入组,共85例,行冠状动脉造影并测量微循环阻力指数(IMR),将患者分为低IMR组48例与高IMR组37例。选择同期在体检中心做健康体检的35名健康者作对照组。对各组研究对象分别测定血清miR-155的相对表达量、IL-6、IL-8、超氧化物歧化酶(SOD)水平,进行统计学分析。结果高IMR组患者血清SOD水平(129.67±12.09)明显低于低IMR组和对照组(分别为144.53±14.89和168.37±15.17,P<0.05),血清miR-155相对表达量、IL-6、IL-8水平分别为0.90±0.13、131.77±10.62和21.35±3.92,明显高于低IMR组和对照组(分别为0.82±0.09、118.32±13.94、17.58±3.01和0.65±0.08、104.58±12.75、14.83±2.77,P<0.05)。高IMR组和低IMR组患者血清miR-155相对表达量与SOD水平呈负相关,与IL-6、IL-8水平呈正相关;多因素logistic回归分析显示,血清miR-155相对表达量增高、SOD水平降低、IL-6水平升高、IL-8水平升高均为X综合征患者冠脉微循环障碍的独立危险因素。结论MiR-155调节氧化应激相关因子的表达,可能是影响X综合征患者冠脉微循环阻力的重要机制之一。Objective To investivate the relationship of microRNA-155(miR-155)and factors related to oxidative stress with coronary microcirculation resistance in patients with syndrome X.Metheds Data were collected from patients with syndrome X hospitalized in Guangzhou First People′s Hospital from January 2018 to December 2020.Coronary angiography was performed and index of Microcirculation resistance(IMR)was measured.Patients were divided into low IMR group(n=48)and high IMR group(n=37).35 healthy subjects in the physical examination center were selected as the control group.The relative expression levels of miR-155,interleukin-6(IL-6),interleukin-8(IL-8)and superoxide dismutase(SOD)in serum of each group were tested respectively.Statistical analysis was performed.Results The level of SOD in high IMR group was significantly lower than that in low IMR group and control group(129.67±12.09 vs.144.53±14.89 and168.37±15.17,P<0.05).The levels of miR-155,IL-6 and IL-8 were markedly higher in high-IMR group than those in lowIMR group and control group(0.90±0.13 vs.0.82±0.09 and 0.65±0.08,P<0.05;131.77±10.62 vs.118.32±13.94 and 104.58±12.75,P<0.05;21.35±3.92 vs.17.58±3.01 and 14.83±2.77,P<0.05,respectively).The relative expression level of miR-155in serum of patients with high IMR and low IMR was negatively correlated with SOD level,was positively correlated with IL-6 and IL-8 levels.Multivariate logistic regression analysis revealed that increased relative expression of miR-155,decreased SOD level,increased IL-6 level and increased IL-8 level were independent risk factors for coronary microcirculation disorders in patients with syndrome X.Conclusion MiR-155 regulates the expression of oxidative stress-related factors,which may be one of the important mechanisms to induce coronary microcirculation disorders in patients with syndrome X.

关 键 词：X综合征 冠状动脉微循环阻力 微小RNA-155 白细胞介素-6 超氧化物歧化酶 

分 类 号：R541.4[医药卫生—心血管疾病]