拟南芥中过表达野菊miR396a基因增强其耐盐性  

Overexpression of Chrysanthemum indicum miR396a Gene in Arabidopsis Enhances Its Salt Tolerance

作　　者：刘筱玮 夏斌[1] 李子葳 杨宇佳 陈斌 周蕴薇 何淼[1] LIU Xiaowei;XIA Bin;LI Ziwei;YANG Yujia;CHEN Bin;ZHOU Yunwei;HE Miao(College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China;College of Horticulture,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]东北林业大学园林学院,哈尔滨150040 [2]吉林农业大学园艺学院,长春130118

出　　处：《园艺学报》2022年第4期816-826,共11页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2018YFD1000406,2019YFD1001504);国家自然科学基金项目(31870687)。

摘　　要：从野菊(Chrysanthemum indicum)中克隆了cin-miR396a前体序列及其启动子,对前体序列进行比对与进化分析,对启动子顺式作用元件进行分析,并研究了cin-miR396a前体序列对拟南芥的遗传转化及对其耐盐性的影响。结果表明,cin-MIR396a长度为145 bp,含有完整的茎环结构,与拟南芥ath-MIR396a进化关系最近。启动子区具有光信号、茉莉酸甲酯、赤霉素、水杨酸、脱落酸等多种响应元件,推测cin-MIR396a参与多种逆境胁迫下的应答及调控。在拟南芥中过表达cin-miR396a前体可以提高盐胁迫下种子发芽率以及成苗期的耐盐性,cin-miR396a在拟南芥响应盐胁迫中发挥正调控作用。cin-miR396a precursor sequence and its promoter were cloned from Chrysanthemum indicum;the precursor sequence alignment and evolutionary analysis were performed;the promoter was analyzed for cis-acting elements.The genetic transformation of the cin-miR396a precursor sequence to Arabidopsis thaliana was further carried out,and the salt tolerance of transgenic Arabidopsis thaliana was analyzed.The results showed that cin-MIR396a was 145 bp in length,contained an intact stem-loop structure,and was evolutionarily closest to A.thaliana ath-MIR396a.The promoter region has multiple response elements such as light signal,methyl jasmonate,gibberellin,salicylic acid,and abscisic acid,and it is hypothesized that cin-MIR396a is involved in response and regulation under a variety of adversity stresses.Overexpression of the cin-miR396a precursor in A.thaliana can improve seed germination under salt stress and salt tolerance at the adult seedling stage,and cin-miR396a plays a positive regulatory role in A.thaliana in response to salt stress.

关 键 词：野菊 miR396 拟南芥 转基因 盐胁迫 

分 类 号：S682.11[农业科学—观赏园艺]