In situ synthesis of red fluorescent gold nanoclusters with enzyme-like activity for oxidative stress amplification in chemodynamic therapy  

作　　者：Wenying Mi Shuang Tang Shaoshi Guo Hejing Li Na Shao 

机构地区：[1]College of Chemistry,Beijing Normal University,Beijing 100875,China

出　　处：《Chinese Chemical Letters》2022年第3期1331-1336,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 22074007)。

摘　　要：Chemodynamic therapy (CDT) has attracted tremendous interest in cancer therapy because it is independent of oxygen and photoirradiation. However, the therapeutic efficacy of CDT is restricted by insufficient H_(2)O_(2) levels in tumor cells. Herein, employing endogenous GSH as a template and cationic polymeric chitosan (CS) as crosslinker and stabilizer exhibiting easy cell uptake, red luminescent gold nanoclusters (denoted CS-GSH@AuNCs) were successfully synthesized in HeLa cells. The in situ synthesized CS-GSH@AuNCs exhibited both superoxidase dismutase (SOD) and peroxidase (POD)-like activity, which could promote the production of H_(2)O_(2) from superoxide anion radicals (O_(2)^(·-)) and then ^(·)OH. The combination of GSH elimination and H_(2)O_(2) elevation boosted the generation of ^(·)OH, which could trigger cancer cell apoptosis and death. The enzyme-like activity of CS-GSH@AuNCs could be effectively activated under acidic conditions, and showed a high killing effect on tumor cells but minimal toxicity to normal cells. The developed GSH consumption and ^(·)OH promotion theranostic platform is an innovative route for enhanced CDT by the amplification of oxidative stress.

关 键 词：Chemodynamic therapy In situ synthesis Gold nanoclusters Superoxidase dismutase and peroxidase(POD)-like activity Oxidative stress amplification 

分 类 号：TB383.1[一般工业技术—材料科学与工程] O614.123[理学—无机化学] R730.5[理学—化学]